Effect of inoculum size on growth of Atropa belladonna hairy roots in shake flasks

被引：21

作者：

Kanokwaree, K ^{[1
]}

Doran, PM ^{[1
]}

机构：

[1] Univ New S Wales, Dept Biotechnol, Sydney, NSW 2052, Australia

来源：

JOURNAL OF FERMENTATION AND BIOENGINEERING | 1997年 / 84卷 / 04期

基金：

澳大利亚研究理事会;

关键词：

Atropa belladonna; hairy roots; inoculum size; medium conditioning; oxygen mass transfer;

D O I：

10.1016/S0922-338X(97)89266-5

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

The initial specific growth rate of Atropa belladonna hairy roots in shake flasks was found to vary with the number of inoculum root tips. Increasing the number of tips from 3 to 9 reduced growth rates in 50 and 100 ml of medium by up to 40%. These results are interpreted by considering the influence of factors such as medium conditioning and oxygen mass transfer on root growth.

引用

页码：378 / 381

页数：4

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