Th1 transmigration anergy:: a new concept of endothelial cell-T cell regulatory interaction

被引:8
作者
Kawai, T
Seki, M
Watanabe, H
Eastcott, JW
Smith, DJ
Taubman, MA
机构
[1] Forsyth Inst, Dept Immunol, Boston, MA 02115 USA
[2] Mitsubishi Tokyo Pharmaceut, Aoba Ku, Yokohama, Kanagawa 227, Japan
[3] Tokyo Med & Dent Univ, Dept Periodontol, Bunkyo Ku, Tokyo 113, Japan
关键词
anergy; B7; MHC class II; T cell migration; vascular endothelium;
D O I
10.1093/intimm/12.6.937
中图分类号
R392 [医学免疫学]; Q939.91 [免疫学];
学科分类号
100102 ;
摘要
Stimulation of endothelial cells (EC) with IFN-gamma generates selective enhancement of T(h)1 cell transmigration and Induction of MHC class II expression on EC, In the present study, we tested whether antigen presentation by EC could influence transmigrating T cells in an in vitro system, Bacterial antigen presentation by EC from primary culture and after cloning induced antigen-specific anergy of transmigrating T(h)1 clone cells in a MHC class Ii-dependent manner as characterized by non-responsiveness to subsequent antigen presentation and inability to produce IL-2, This T cell transmigration anergy induced by EC was abrogated by anti-rat CD28 mAb, suggesting that lack of B7 co-stimulatory signals by EC might be related to the induction of anergy, While MHC class II expression on primary and cloned EC was observed after IFN-gamma stimulation, these cells never expressed B7, B7-1 gene-transfected endothelial clone cells (ECC/B7-1) were developed to elucidate the influence of B7 co-stimulation by EC, ECC/B7-1 induced proliferation of T(h)1 clone cells, whereas ECC did not induce proliferation in co-culture of T(h)1 clone cells and EC stimulated with IFN-gamma and antigen. in the transmigration assay, ECC/B7-1 did not induce transmigration anergy of T(h)1 clones or T(h)1 lines unless anti-rat B7-1 blocking mAb was added. Therefore, in rats, the T cell anergy induced during transmigration across a layer of EC seemed to be due to antigen presentation in the absence of B7 on the EC. We introduce the concept of transmigration anergy In this manuscript. Thus, EC can play a critical immune regulatory role in the context of antigen presentation by MHC class II to transmigrating T cells.
引用
收藏
页码:937 / 948
页数:12
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