Structure-activity analysis of the interaction of curacin A, the potent colchicine site antimitotic agent, with tubulin and effects of analogs on the growth of MCF-7 breast cancer cells

被引:252
作者
Verdier-Pinard, P
Lai, JY
Yoo, HD
Yu, JR
Marquez, B
Nagle, DG
Nambu, M
White, JD
Falck, JR
Gerwick, WH
Day, BW
Hamel, E
机构
[1] NCI, Lab Drug Discovery Res & Dev, Dev Therapeut Program,Div Canc Treatment & Diag, Frederick Canc Res & Dev Ctr, Frederick, MD 21702 USA
[2] Univ Texas, SW Med Ctr, Dept Biochem, Dallas, TX 75235 USA
[3] Univ Texas, SW Med Ctr, Dept Pharmacol, Dallas, TX 75235 USA
[4] Oregon State Univ, Coll Pharm, Corvallis, OR 97331 USA
[5] Oregon State Univ, Dept Chem, Corvallis, OR 97331 USA
[6] Univ Pittsburgh, Inst Canc, Dept Environm & Occupat Hlth, Pittsburgh, PA 15238 USA
[7] Univ Pittsburgh, Inst Canc, Dept Pharmaceut Sci, Pittsburgh, PA 15238 USA
关键词
D O I
10.1124/mol.53.1.62
中图分类号
R9 [药学];
学科分类号
1007 ;
摘要
Originally purified as a major lipid component of a strain of the cyanobacterium Lyngbya majuscula isolated in Curacao, curacin A is a potent inhibitor of cell growth and mitosis, binding rapidly and tightly at the colchicine site of tubulin. Because its molecular structure differs so greatly from that of colchicine and other colchicine site inhibitors, we prepared a series of curacin A analogs to determine the important structural features of the molecule. These modifications include reduction and E-to-Z transitions of the olefinic bonds in the 14-carbon side chain of the molecule; disruption of and configurational changes in the cyclopropyl moiety; disruption, oxidation, and configurational reversal in the thiazoline moiety; configurational reversal and substituent modifications at C13; and demethylation at C10. Inhibitory effects on tubulin assembly, the binding of colchicine to tubulin, and the growth of MCF-7 human breast carcinoma cells were examined. The most important portions of curacin A required for its interaction with tubulin seem to be the thiazoline ring and the side chain at least through C4, the portion of the side chain including the C9-10 olefinic bond, and the C10 methyl group. Only two modifications totally eliminated the tubulin-drug interaction. The inactive compounds were a segment containing most of the side chain, including its two substituents, and analogs in which the methyl group at the C13 oxygen atom was replaced by a benzoate residue. Antiproliferative activity comparable with that observed with curacin A was only reproduced in compounds that were potent inhibitors of the binding of colchicine to tubulin. Molecular modeling and quantitative structure-activity relationship studies demonstrated that most active analogs overlapped extensively with curacin A but failed to provide an explanation for the apparent structural analogy between curacin A and colchicine.
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页码:62 / 76
页数:15
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