Reversible Ponceau staining as a loading control alternative to actin in Western blots

被引：650

作者：

Romero-Calvo, Isabel

Ocon, Borja

Martinez-Moya, Patricia

Dolores Suarez, Maria

Zarzuelo, Antonio

Martinez-Augustin, Olga

Sanchez de Medina, Fermin ^{[1
]}

机构：

[1] Univ Granada, Sch Pharm, CIBERehd, Dept Biochem, E-18071 Granada, Spain

来源：

ANALYTICAL BIOCHEMISTRY | 2010年 / 401卷 / 02期

关键词：

Proteins;

D O I：

10.1016/j.ab.2010.02.036

中图分类号：

Q5 [生物化学];

学科分类号：

070307 [化学生物学];

摘要：

It is becoming standard practice to measure a housekeeping gene, typically actin, in Western blots, as it is the rule in RNA blots. We have applied reversible Ponceau staining to check equal loading of gels and measured actin in parallel under different conditions. Our results show that densitometric analysis is comparable with both techniques. Therefore, routine quantitation of Ponceau staining before antibody probing is validated as an alternative to actin blotting. (C) 2010 Elsevier Inc. All rights reserved.

引用

页码：318 / 320

页数：3

共 5 条

[1]

The use of total protein stains as loading controls: An alternative to high-abundance single-protein controls in semi-quantitative immunoblotting [J].