Fragilysin, the enterotoxin from Bacteroides fragilis, enhances the serum antibody response to antigen co-administered by the intranasal route

被引：16

作者：

Vines, RR ^{[1
]}

Perdue, SS ^{[1
]}

Moncrief, JS ^{[1
]}

Sentz, DR ^{[1
]}

Barroso, LA ^{[1
]}

Wright, RL ^{[1
]}

Wilkins, TD ^{[1
]}

机构：

[1] Virginia Polytech Inst & State Univ, Dept Biochem, Fralin Biotechnol Ctr, Blacksburg, VA 24061 USA

来源：

VACCINE | 2000年 / 19卷 / 06期

关键词：

enterotoxigenic Bacteroides fragilis; fragilysin; intranasal adjuvant;

D O I：

10.1016/S0264-410X(00)00254-1

中图分类号：

R392 [医学免疫学]; Q939.91 [免疫学];

学科分类号：

100102 ;

摘要：

Fragilysin, an extracellular zinc metalloprotease produced by enterotoxigenic strains of the anaerobic bacterium Bacteroides fragilis, disrupts the paracellular barrier by cleavage of the intercellular proteins between epithelial cells resulting in fluid secretion. Intranasal immunization of mice with fragilysin and co-administered ovalbumin (Ova) resulted in an Ova-specific serum IgG response that was over 18 000-fold higher than Ova alone, as well as detectable levels of serum IgA. Serum IgG titers were comparable with those seen when whole cholera toxin was used as the adjuvant, although the responses obtained with fragilysin showed more variability between mice, Metalloproteases to which fragilysin is structurally related were ineffective as mucosal adjuvants. Our results and similar studies with enterotoxins that affect the paracellular barrier suggest that alteration of mucosal permeability may Flay an important role in the mechanisms of adjuvanticity. (C) 2000 Elsevier Science Ltd. All rights reserved.

引用

页码：655 / 660

页数：6

共 37 条

[1] TIGHT JUNCTIONS AND THE MOLECULAR-BASIS FOR REGULATION OF PARACELLULAR PERMEABILITY [J].