Integrated bombardment and Agrobacterium transformation system:: an alternative method for soybean transformation

被引：21

作者：

Droste, A

Pasquali, G

Bodanese-Zanettini, MH

机构：

[1] Univ Vale do Rio dos Sinos, Ctr Ciencias Saude, Setor Bot, BR-93022000 Sao Leopoldo, RS, Brazil

[2] Univ Fed Rio Grande Sul, Ctr Biotecnol Estado Rio Grande Sul, BR-91501970 Porto Alegre, RS, Brazil

[3] Univ Fed Rio Grande Sul, Inst Biociencias, Dept Biotecnol, BR-91501970 Porto Alegre, RS, Brazil

[4] Univ Fed Rio Grande Sul, Inst Biociencias, Dept Genet, BR-91501970 Porto Alegre, RS, Brazil

来源：

PLANT MOLECULAR BIOLOGY REPORTER | 2000年 / 18卷 / 01期

关键词：

Agrobacterium; Glycine max; particle bombardment; somatic embryogenesis; transformation;

D O I：

10.1007/BF02825294

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

Described here is a new method for soybean transformation, based on micro-wounding of embryonic clumps by particle bombardment prior to inoculation with an Agrobacterium suspension. The method combines the advantages of somatic embryogenesis and gene transfer through an integrated transformation system. This is the first report of application of this technique to transformation of Glycine max.

引用

页码：51 / 59

页数：9

共 29 条

[1]

BAILEY MA, 1993, IN VITRO CELL DEV-PL, V29P, P102

[2] MICROPROJECTILE BOMBARDMENT OF PLANT-TISSUES INCREASES TRANSFORMATION FREQUENCY BY AGROBACTERIUM-TUMEFACIENS [J].