Neuroprotection in a rat Parkinson model by GDNF gene therapy using EIAV vector

被引：43

作者：

Azzouz, M ^{[1
]}

Ralph, S ^{[1
]}

Wong, LF ^{[1
]}

Day, D ^{[1
]}

Askham, Z ^{[1
]}

Barber, RD ^{[1
]}

Mitrophanous, KA ^{[1
]}

Kingsman, SM ^{[1
]}

Mazarakis, ND ^{[1
]}

机构：

[1] Oxford BioMed UK Ltd, Oxford OX4 4GA, England

来源：

NEUROREPORT | 2004年 / 15卷 / 06期

关键词：

EIAV lentiviral vectors; GDNF; 6-OHDA partial lesion; Parkinson's disease;

D O I：

10.1097/00001756-200404290-00011

中图分类号：

Q189 [神经科学];

学科分类号：

071006 ;

摘要：

Vectors based on lentiviruses are opening up new approaches for the treatment of neurodegenerative diseases. Currently, the equine infectious anaemia virus (EIAV) vector is one of the most attractive gene delivery systems with respect to neuronal tropism. The aim was to validate EIAV-lentiviral vectors as a gene delivery system for neurotrophic factor genes in an animal model of Parkinson's disease. EIAV carrying the glial cell line-derived neurotrophic factor (GDNF) gene was unilaterally injected into rat striatum and above the substantia nigra (SN). One week later, the rats received a 6-OHDA lesion into the ipsilateral striatum. GDNF delivery led to extensive expression of GDNF protein within the striatum. In addition, near complete protection against dopaminergic cell death was observed in the GDNF-treated group.

引用

收藏

页码：985 / 990

页数：6

相关论文

共 25 条

[1]

Azzouz M, 2002, J NEUROSCI, V22, P10302

[2] Lentiviral vectors as a gene delivery system in the mouse midbrain:: Cellular and behavioral improvements in a 6-OHDA model of Parkinson's disease using GDNF [J].

Bensadoun, JC ;

Déglon, N ;

Tseng, JL ;

Ridet, JL ;

Zurn, AD ;

Aebischer, P .

EXPERIMENTAL NEUROLOGY, 2000, 164 (01) :15-24

[3] Long-term replacement of a mutated nonfunctional CNS gene: Reversal of hypothalamic diabetes insipidus using an EIAV-based lentiviral vector expressing arginine vasopressin [J].

Bienemann, AS ;

Martin-Rendon, E ;

Cosgrave, AS ;

Glover, CPJ ;

Wong, LF ;

Kingsman, SM ;

Mitrophanous, KA ;

Mazarakis, ND ;

Uney, JB .

MOLECULAR THERAPY, 2003, 7 (05) :588-596

[4] Towards a neuroprotective gene therapy for Parkinson's disease:: use of adenovirus, AAV and lentivirus vectors for gene transfer of GDNF to the nigrostriatal system in the rat Parkinson model [J].

Björklund, A ;

Kirik, D ;

Rosenblad, C ;

Georgievska, B ;

Lundberg, C ;

Mandel, RJ .

BRAIN RESEARCH, 2000, 886 (1-2) :82-98

[5] A NUCLEAR-LOCALIZATION SIGNAL WITHIN HIV-1 MATRIX PROTEIN THAT GOVERNS INFECTION OF NONDIVIDING CELLS [J].

BUKRINSKY, MI ;

HAGGERTY, S ;

DEMPSEY, MP ;

SHAROVA, N ;

ADZHUBEI, A ;

SPITZ, L ;

LEWIS, P ;

GOLDFARB, D ;

EMERMAN, M ;

STEVENSON, M .

NATURE, 1993, 365 (6447) :666-669

[6] Dopaminergic neurons protected from degeneration by GDNF gene therapy [J].

ChoiLundberg, DL ;

Lin, Q ;

Chang, YN ;

Chiang, YL ;

Hay, CM ;

Mohajeri, H ;

Davidson, BL ;

Bohn, MC .

SCIENCE, 1997, 275 (5301) :838-841

[7] Viral vectors for gene delivery to the nervous system [J].

Davidson, BL ;

Breakefield, XO .

NATURE REVIEWS NEUROSCIENCE, 2003, 4 (05) :353-364

[8]

Dèglon N, 2002, CURR TOP MICROBIOL, V261, P191

[9] Self-inactivating lentiviral vectors with enhanced transgene expression as potential gene transfer system in Parkinson's disease [J].

Déglon, N ;

Tseng, JL ;

Bensadoun, JC ;

Zurn, AD ;

Arsenijevic, Y ;

De Almeida, LP ;

Zufferey, R ;

Trono, D ;

Aebischer, P .

HUMAN GENE THERAPY, 2000, 11 (01) :179-190

[10] Neuroprotection in the rat Parkinson model by intrastriatal GDNF gene transfer using a lentiviral vector [J].

Georgievska, B ;

Kirik, D ;

Rosenblad, C ;

Lundberg, C ;

Björklund, A .

NEUROREPORT, 2002, 13 (01) :75-82