PCR-restriction fragment length polymorphism assay for detection of gyrA mutations associated with fluoroquinolone resistance in Campylobacter coli

被引：20

作者：

Alonso, R ^{[1
]}

Mateo, E ^{[1
]}

Girbau, C ^{[1
]}

Churruca, E ^{[1
]}

Martinez, I ^{[1
]}

Fernández-Astorga, A ^{[1
]}

机构：

[1] Univ Basque Country, UPV EHU, Fac Farm, Dept Inmunol Microbiol & Parasitol, Vitoria 01006, Spain

来源：

ANTIMICROBIAL AGENTS AND CHEMOTHERAPY | 2004年 / 48卷 / 12期

关键词：

D O I：

10.1128/AAC.48.12.4886-4888.2004

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

A fragment of the gyrA gene was sequenced from 34 isolates of Campylobacter coli, including 23 isolates resistant to ciprofloxacin. All ciprofloxacin-resistant isolates examined by DNA sequencing carried a point mutation at position Thr-86 on the gyrA gene product, involving the replacement of Thr-86 by Ile. A combined PCR-restriction fragment length polymorphism technique using RsaI was developed to detect this mutation.

引用

收藏

页码：4886 / 4888

页数：3

相关论文

共 19 条

[1] Single or double mutational alterations of GyrA associated with fluoroquinolone resistance in Campylobacter jejuni and Campylobacter coli [J].

Bachoual, R ;

Ouabdesselam, S ;

Mory, F ;

Lascols, C ;

Soussy, CJ ;

Tankovic, J .

MICROBIAL DRUG RESISTANCE, 2001, 7 (03) :257-261

[2]

BLASER MJ, 2000, BENNETTS PRINCIPLES, P2276

[3] QUINOLONE RESISTANCE IN CAMPYLOBACTER ISOLATED FROM MAN AND POULTRY FOLLOWING THE INTRODUCTION OF FLUOROQUINOLONES IN VETERINARY-MEDICINE [J].

ENDTZ, HP ;

RUIJS, GJ ;

VANKLINGEREN, B ;

JANSEN, WH ;

VANDERREYDEN, T ;

MOUTON, RP .

JOURNAL OF ANTIMICROBIAL CHEMOTHERAPY, 1991, 27 (02) :199-208

[4] Quinolone and macrolide resistance in Campylobacter jejuni and C-coli:: Resistance mechanisms and trends in human isolates [J].

Engberg, J ;

Aarestrup, FM ;

Taylor, DE ;

Gerner-Smidt, P ;

Nachamkin, I .

EMERGING INFECTIOUS DISEASES, 2001, 7 (01) :24-34

[5]

Friedman C.R., 2000, CAMPYLOBACTER, V2nd, P121

[6] Antimcrobial-resistant Campylobacter species from retail raw meats [J].

Ge, BL ;

White, DG ;

McDermott, PF ;

Girard, W ;

Zhao, SH ;

Hubert, S ;

Meng, JH .

APPLIED AND ENVIRONMENTAL MICROBIOLOGY, 2003, 69 (05) :3005-3007

[7] CHARACTERIZATION OF HIGH-LEVEL QUINOLONE RESISTANCE IN CAMPYLOBACTER-JEJUNI [J].

GOOTZ, TD ;

MARTIN, BA .

ANTIMICROBIAL AGENTS AND CHEMOTHERAPY, 1991, 35 (05) :840-845

[8] Molecular typing of Campylobacter jejuni isolates involved in a neonatal outbreak indicates nosocomial transmission [J].

Llovo, J ;

Mateo, E ;

Muñoz, A ;

Urquijo, M ;

On, SLW ;

Fernández-Astorga, A .

JOURNAL OF CLINICAL MICROBIOLOGY, 2003, 41 (08) :3926-3928

[9] Antimicrobial resistance in Campylobacter jejuni and Campylobacter coli strains isolated in 1991 and 2001-2002 from poultry and humans in Berlin, Germany [J].

Luber, P ;

Wagner, J ;

Hahn, H ;

Bartelt, E .

ANTIMICROBIAL AGENTS AND CHEMOTHERAPY, 2003, 47 (12) :3825-3830

[10]

*NAT COMM CLIN LAB, 2002, M7A5 NAC COMM CLIN L, V22