Quantification of platelets and platelet derived growth factors from platelet-rich-plasma (PRP) prepared at different centrifugal force (g) and time

Introduction: Platelet derived biomaterials represent a key source of cytokines and growth factors extensively used for tissue regeneration; wound healing and tissue repair. Our study was to quantify platelets and growth factors released by PRP when prepared at different centrifugal force (g) and time. Material and Methods: Our study was approved by the institutional ethical committee. One hundred millilitres of whole blood (WB) was collected in bag with CPDA as the anticoagulant(AC); (14 mL for 100 mL WB ratio). Nine aliquots of 10 mL each were made from the bag and set of three aliquots were made a group. PRP was prepared at varying centrifugal force (group A:-110 g, group B:-208 g & group C:-440 g) & time (1:-5 min, 2:-10 min & 3:-20 min). Contents of each PRP prepared were analysed. Commercial sandwich ELISA kits were used to quantify the concentrations of CD62P (Diaclone SAS; France), Platelet derived growth factors-AB (Qayee-Bio; China), transforming growth factor-beta 1 (DRG; Germany) and vascular endothelial growth factor (Boster Immuno Leader; USA) released in each PRP prepared. Results: Eight volunteers were enrolled in the study (24-30 years). The baseline blood counts of all the volunteers were comparable (p >= 0.05). Mean +/- SD of platelet yield of all nine groups ranged from 17.2 +/- 4.2% to 78.7 +/- 5.7%. Each PRP was activated with calcified thromboplastin to quantify the growth factors released by them. Significantly higher (p <0.05) transforming growth factor-beta 1 and vascular endothelial growth factor were released compared to the baseline. Conclusion: Our study highlights the variation in both force (g) and time results in changes at cellular level and growth factor concentrations. (c) 2016 Elsevier Ltd. All rights reserved.