Induction of hydroxyanthranilate hydroxycinnamoyl transferase activity by oligo-N-acetylchitooligosaccharides in oats

被引：42

作者：

Ishihara, A ^{[1
]}

Miyagawa, H

Matsukawa, T

Ueno, T

Mayama, S

Iwamura, H

机构：

[1] Kyoto Univ, Dept Agr Chem, Kyoto 60601, Japan

[2] Kobe Univ, Fac Agr, Kobe, Hyogo 657, Japan

[3] Japan Sci & Technol Corp, CREST, Kobe, Hyogo 657, Japan

来源：

PHYTOCHEMISTRY | 1998年 / 47卷 / 06期

关键词：

Avena sativa; gramineae; avenanthramide; oats; anthranilic acid; hydroxycinnamoyl-CoA; biosynthesis;

D O I：

10.1016/S0031-9422(97)00603-1

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

An assay method for hydroxycinnamoyl-CoA: hydroxyanthranilate N-hydroxycinnamoyl transferase (HHT) in oat leaves (Avena sativa L.), which is thought to be one of the key enzymes for the biosynthesis of avenanthramides, phytoalexins in this plant, was established. HHT activity was induced by treating the leaves with oligo-N-acetylchitooligosaccharides. Among the chitooligosaccharides tested, penta-N-acetylchitopentaose ((GlcNAc)(5)) was the most effective in inducing activity. The induction by (GlcNAc)(5) was dose-dependent, in which case HHT activity was initially detected after 6 hr and reached a maximum by 12 hr. All of the putative precursors of avenanthramides acted as substrates for HHT, with 5-hydroxyanthranilic acid and feruloyl-CoA being the best substrates for the anthranilic moiety and the cinnamoyl moiety of avenanthramides, respectively. (C) 1998 Elsevier Science Ltd. All rights reserved.

引用

页码：969 / 974

页数：6

共 22 条

[1] CELL WALL CHEMISTRY MORPHOGENESIS AND TAXONOMY OF FUNGI [J].