Molecular characterisation of Plasmodium reichenowi apical membrane antigen-1 (AMA-1), comparison with P-falciparum AMA-1, and antibody-mediated inhibition of red cell invasion

被引:71
作者
Kocken, CHM
Narum, DL
Massougbodji, A
Ayivi, B
Dubbeld, MA
van der Wel, A
Conway, DJ
Sanni, A
Thomas, AW
机构
[1] Biomed Primate Res Ctr, Dept Parasitol, NL-2280 GH Rijswijk, Netherlands
[2] Natl Hosp & Univ CNHU, Parasitol Ctr, Cotonou, Benin
[3] Univ London London Sch Hyg & Trop Med, Dept Infect & Trop Dis, London WC1E 7HT, England
[4] UNB, Fac Sci & Tech, Dept Biochim & Biol Cellulaire, Cotonou, Benin
关键词
malaria; Plasmodium reichenowi; Plasmodium falciparum; apical membrane antigen-1; polymorphism; red cell invasion;
D O I
10.1016/S0166-6851(00)00250-4
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
Apical membrane antigen 1 is a candidate vaccine component for malaria. It is encoded by a single copy gene and has been characterised in a number of malaria species as either an 83-kDa de novo product (Plasmodium falciparum: Pf AMA-1) or a 66-kDa product (all other species). All members of the AMA-1 family are expressed during merozoite formation in maturing schizonts and are initially routed to the rhoptries. Processed forms may subsequently be associated with the merozoite surface. Because of the unique occurrence of the 83-kDa form in P. falciparum we were interested to determine whether the phylogenetically closely related chimpanzee malaria plasmodium reichenowi shared characteristics with Pf AMA-1. Here we show that the molecular structure, the localisation and processing are similar to that of Pf AMA-1 and that in vitro growth inhibitory mAbs reactive with Pf AMA-1 also inhibit P. reichenowi growth in an in vitro assay. Polymorphism in the 83-kDa AMA-1 family was analysed through comparison of Pr ama-1 with Pf ama-1 alleles, which showed the most significant evidence for selection maintaining polymorphism in Domains I-III of AMA-1 in P. falciparum. The most substantial divergence between Pr AMA-1 and Pf AMA-1 sequences was in the N-terminal region unique to the 83-kDa form of AMA-1. It was confirmed that the specific Pr ama-1-type allele was not present among P. falciparum parasites in an African population, and an allele coding for lysine at amino acid 187 was uniquely associated with field isolates in this population. (C) 2000 Elsevier Science B.V. All rights reserved.
引用
收藏
页码:147 / 156
页数:10
相关论文
共 29 条
  • [1] Immunisation with recombinant AMA-1 protects mice against infection with Plasmodium chabaudi
    Anders, RF
    Crewther, PE
    Edwards, S
    Margetts, M
    Matthew, MLSM
    Pollock, B
    Pye, D
    [J]. VACCINE, 1998, 16 (2-3) : 240 - 247
  • [2] PROTECTIVE IMMUNITY INDUCED IN SQUIRREL-MONKEYS WITH RECOMBINANT APICAL MEMBRANE ANTIGEN-1 OF PLASMODIUM FRAGILE
    COLLINS, WE
    PYE, D
    CREWTHER, PE
    VANDENBERG, KL
    GALLAND, GG
    SULZER, AJ
    KEMP, DJ
    EDWARDS, SJ
    COPPEL, RL
    SULLIVAN, JS
    MORRIS, CL
    ANDERS, RF
    [J]. AMERICAN JOURNAL OF TROPICAL MEDICINE AND HYGIENE, 1994, 51 (06) : 711 - 719
  • [3] Protective immune responses to apical membrane antigen 1 of Plasmodium chabaudi involve recognition of strain-specific epitopes
    Crewther, PE
    Matthew, MLSM
    Flegg, RH
    Anders, RF
    [J]. INFECTION AND IMMUNITY, 1996, 64 (08) : 3310 - 3317
  • [4] DEANS JA, 1982, CLIN EXP IMMUNOL, V49, P297
  • [5] VACCINATION TRIALS IN RHESUS-MONKEYS WITH A MINOR, INVARIANT, PLASMODIUM-KNOWLESI 66 KD MEROZOITE ANTIGEN
    DEANS, JA
    KNIGHT, AM
    JEAN, WC
    WATERS, AP
    COHEN, S
    MITCHELL, GH
    [J]. PARASITE IMMUNOLOGY, 1988, 10 (05) : 535 - 552
  • [6] A Plasmodium falciparum epical membrane antigen-1 (AMA-1) gene apparently generated by intragenic recombination
    Eisen, DP
    Marshall, VM
    Billman-Jacobe, H
    Coppel, RL
    [J]. MOLECULAR AND BIOCHEMICAL PARASITOLOGY, 1999, 100 (02) : 243 - 246
  • [7] Escalante AA, 1998, GENETICS, V149, P189
  • [8] The evolution of primate malaria parasites based on the gene encoding cytochrome b from the linear mitochondrial genome
    Escalante, AA
    Freeland, DE
    Collins, WE
    Lal, AA
    [J]. PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1998, 95 (14) : 8124 - 8129
  • [9] PHYLOGENY OF THE MALARIAL GENUS PLASMODIUM, DERIVED FROM RIBOSOMAL-RNA GENE-SEQUENCES
    ESCALANTE, AA
    AYALA, FJ
    [J]. PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1994, 91 (24) : 11373 - 11377
  • [10] MANS PLACE IN HOMINOIDEA AS INFERRED FROM MOLECULAR CLOCKS OF DNA
    HASEGAWA, M
    KISHINO, H
    YANO, TA
    [J]. JOURNAL OF MOLECULAR EVOLUTION, 1987, 26 (1-2) : 132 - 147