Mammalian PIG-X and yeast Pbn1p are the essential components of glycosylphosphatidylinositol-mannosyltransferase I

被引:51
作者
Ashida, H
Hong, YJ
Murakami, Y
Shishioh, N
Sugimoto, N
Kim, YU
Maeda, Y
Kinoshita, T [1 ]
机构
[1] Osaka Univ, Res Inst Microbial Dis, Dept Immunoregulat, Suita, Osaka 5650871, Japan
[2] Chonnam Natl Univ, Sch Med, Genom Res Ctr Enteropathogen Bacteria, Kwangju, South Korea
[3] Chonnam Natl Univ, Sch Med, Dept Microbiol, Kwangju, South Korea
[4] Osaka Univ, Grad Sch Med, Dept Mol & Appl Med, Div Adv Med Bacteriol, Osaka, Japan
[5] Sunmoon Univ, Div Appl Biol Sci, Asan, South Korea
关键词
D O I
10.1091/mbc.E04-09-0802
中图分类号
Q2 [细胞生物学];
学科分类号
071009 ; 090102 ;
摘要
Within the endoplasmic reticulum (ER), mannoses and glucoses, donated from dolichol-phosphate-mannose and -glucose, are transferred to N-glycan and GPI-anchor precursors, and serine/threonine residues in many proteins. Glycosyltransferases that mediate these reactions are ER-resident multitransmembrane proteins with common characteristics, forming a superfamily of >10 enzymes. Here, we report an essential component of glycosylphosphatidylinositolmannosyltransferase I (GPI-MT-I), which transfers the first of the four mannoses in the GPI-anchor precursors. We isolated a Chinese hamster ovary (CHO) cell mutant defective in GPI-MT-I but not its catalytic component PIG-M. The mutant gene, termed phosphatidylinositolglycan-class X (PIG-X), encoded a 252-amino acid ER-resident type I transmembrane protein with a large lumenal domain. PIG-X and PIG-M formed a complex, and PIG-M expression was <10% in the absence of PIG-X, indicating that PIG-X stabilizes PIG-M. We found that Saccharomyces cerevisiae Pbn1p/YCL052Cp, which was previously reported to be involved in autoprocessing of proproteinase B, is the functional homologue of PIG-X; Pbn1p is critical for Gpi14p/YJR013Wp function, the yeast homologue of PIG-M. This is the first report of an essential subcomponent of glycosyltransferases using dolichol-phosphate-monosaccharide.
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收藏
页码:1439 / 1448
页数:10
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