Spontaneous Aneuploidy and Clone Formation in Adipose Tissue Stem Cells during Different Periods of Culturing

被引：35

作者：

Buyanovskaya, O. A. ^{[1
]}

Kuleshov, N. P. ^{[1
]}

Nikitina, V. A. ^{[2
]}

Voronina, E. S. ^{[2
]}

Katosova, L. D. ^{[2
]}

Bochkov, N. P. ^{[1
]}

机构：

[1] IM Sechenov Moscow Med Acad, Moscow, Russia

[2] Russian Acad Med Sci, Ctr Med Genet, Moscow, Russia

来源：

BULLETIN OF EXPERIMENTAL BIOLOGY AND MEDICINE | 2009年 / 148卷 / 01期

基金：

俄罗斯基础研究基金会;

关键词：

stem cells; aneuploidy; clone formation; interphase nuclei; fluorescent in situ hybridization (FISH);

D O I：

10.1007/s10517-009-0647-3

中图分类号：

R-3 [医学研究方法]; R3 [基础医学];

学科分类号：

1001 ;

摘要：

Cytogenetic analysis of 13 mesenchymal stem cell cultures isolated from normal human adipose tissue was carried out at different stages of culturing. The incidence of chromosomes 6, 8, 11, and X aneuploidy and polyploidy was studied by fl uorescent in situ hybridization. During the early passages, monosomal cells were more often detected than trisomal ones. A clone with chromosome 6 monosomy was detected in three cultures during late passages.

引用

页码：109 / 112

页数：4

共 10 条

[1]

[Бочков Николай Павлович Bochkov N.P.], 2008, [Молекулярная медицина, Molekulyarnaya meditsina], P40

[2]

BOCHKOV NP, 2007, KLETOCH TEKHNOL BIOL, P11

[3] Karyotype of human ES cells during extended culture [J].

Buzzard, JJ ;

Gough, NM ;

Crook, JM ;

Colman, A .

NATURE BIOTECHNOLOGY, 2004, 22 (04) :381-382

[4] DETECTION OF ANEUPLOIDY AND ANEUPLOIDY-INDUCING AGENTS IN HUMAN-LYMPHOCYTES USING FLUORESCENCE INSITU HYBRIDIZATION WITH CHROMOSOME-SPECIFIC DNA PROBES [J].

EASTMOND, DA ;

PINKEL, D .

MUTATION RESEARCH, 1990, 234 (05) :303-318

[5] Genomic alterations in cultured human embryonic stem cells [J].

Maitra, A ;

Arking, DE ;

Shivapurkar, N ;

Ikeda, M ;

Stastny, V ;

Kassauei, K ;

Sui, GP ;

Cutler, DJ ;

Liu, Y ;

Brimble, SN ;

Noaksson, K ;

Hyllner, J ;

Schulz, TC ;

Zeng, XM ;

Freed, WJ ;

Crook, J ;

Abraham, S ;

Colman, A ;

Sartipy, P ;

Matsui, SI ;

Carpenter, M ;

Gazdar, AF ;

Rao, M ;

Chakravarti, A .

NATURE GENETICS, 2005, 37 (10) :1099-1103

[6] Preserving the genetic integrity of human embryonic stem cells [J].

Mitalipova, MM ;

Rao, RR ;

Hoyer, DM ;

Johnson, JA ;

Meisner, LF ;

Jones, KL ;

Dalton, S ;

Stice, SL .

NATURE BIOTECHNOLOGY, 2005, 23 (01) :19-20

[7]

Mizuno Hiroshi, 2003, Journal of Nippon Medical School, V70, P300

[8]

MUSINA RA, 2005, KLETOCHN TEKHNOL BIO, P89

[9]

NAZARENKO SA, 2004, GENETIKA, P195

[10] RETRACTED: Spontaneous human adult stem cell transformation (Retracted article. See vol. 70, pg. 6682, 2010) [J].

Rubio, D ;

Garcia-Castro, J ;

Martín, MC ;

de la Fuente, R ;

Cigudosa, JC ;

Lloyd, AC ;

Bernad, A .

CANCER RESEARCH, 2005, 65 (08) :3035-3039

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