Analysis of phosphorylated carbohydrates by high-performance liquid chromatography electrospray ionization tandem mass spectrometry utilising a β-cyclodextrin bonded stationary phase

被引：44

作者：

Feurle, J

Jomaa, H

Wilhelm, M

Gutsche, B

Herderich, M ^{[1
]}

机构：

[1] Univ Wurzburg, Lehrstuhl Lebensmittelchem, D-97074 Wurzburg, Germany

[2] Univ Wurzburg, Med Poliklin, D-97070 Wurzburg, Germany

来源：

JOURNAL OF CHROMATOGRAPHY A | 1998年 / 803卷 / 1-2期

关键词：

carbohydrates; D-1-deoxyxylulose-5-phosphate; sugar phosphates;

D O I：

10.1016/S0021-9673(97)01214-4

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

Chromatographic separation of phosphorylated carbohydrates was achieved on a beta-cyclodextrin (CD) bonded HPLC column. Applying acetonitrile-aqueous ammonium acetate gradient elution, the separation of sugar phosphates relied on the combination of anion-exchange and hydrophilic interaction properties of CD bonded phases in the presence of ammonium ions. Furthermore, this solvent system allowed on-line coupling to electrospray ionization mass spectrometry (ESI-MS) and direct structural characterisation of carbohydrate phosphates by tandem mass spectrometry (MS-MS). In addition to the analysis of various pentose and hexose phosphates, the HPLC-ESI-MS-MS method was successfully applied to demonstrate the enzymatic formation of D-1-deoxyxylulose 5-phosphate from pyruvate and glyceraldehyde phosphate catalysed by yeast transketolase. (C) 1998 Elsevier Science B.V.

引用

页码：111 / 119

页数：9

共 32 条

[1] EVALUATION OF THE LIQUID-CHROMATOGRAPHIC SEPARATION OF MONOSACCHARIDES, DISACCHARIDES, TRISACCHARIDES, TETRASACCHARIDES, DEOXYSACCHARIDES AND SUGAR ALCOHOLS WITH STABLE CYCLODEXTRIN BONDED PHASE COLUMNS [J].