Biomimetic collagen scaffolds for human bone cell growth and differentiation

被引:82
作者
Yang, XBB
Bhatnagar, RS
Li, S
Oreffo, ROC
机构
[1] Univ Southampton, Bone & Joint Res Grp, Southampton SO16 6YD, Hants, England
[2] Univ Calif San Francisco, Lab Connect Tissue Biochem, San Francisco, CA 94143 USA
[3] Univ Calif Berkeley, Dept Bioengn, Berkeley, CA 94720 USA
来源
TISSUE ENGINEERING | 2004年 / 10卷 / 7-8期
关键词
D O I
10.1089/ten.2004.10.1148
中图分类号
Q813 [细胞工程];
学科分类号
摘要
Type I collagen provides a structural framework for connective tissues and plays a central role in the temporal cascade of events leading to the formation of new bone from progenitors. The aim of this study was to examine the ability of the cell-binding domain of type I collagen (P-15 peptide) to promote human bone marrow stromal cell adhesion, proliferation, and differentiation on three-dimensional scaffolds. Human bone marrow stromal cells were selected, expanded, and cultured on particulate microporous ABM ("pure" hydroxyapatite) phase adsorbed with or without P-15 under basal or osteogenic conditions. Immobilized P-15 increased alkaline phosphatase activity and bone morphogenetic protein 2 (BMP-2) gene expression after 1 and 5 days as determined by real-time polymerase chain reaction. P-15 promoted human bone marrow stromal cell attachment, spreading, and alignment on ABM as well as alkaline phosphatase-specific activity in basal and osteogenic cultures. The presence of mineralized bone matrix, extensive cell ingrowth, and cellular bridging between three-dimensional matrices adsorbed with P-15 was confirmed by confocal microscopy, scanning electron microscopy, and alizarin red staining. Negligible cell growth was observed on ABM alone. In vivo diffusion chamber studies using MF1-nu/nu mice showed bone matrix formation and organized collagen formation after 6 weeks. These studies indicate the potential of P-15 to generate appropriate biomimetic microenvironments for osteoblasts and demonstrate the potential for the exploitation of extracellular matrix cues for osteogenesis and, ultimately, bone regeneration.
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页码:1148 / 1159
页数:12
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