Cytogenetic chromosomal preparations using 2-methoxyestradiol

被引:2
作者
Attalla, H
Knuutila, S
Mäkelä, TP
Andersson, LC
Adlercreutz, H
机构
[1] Univ Helsinki, Dept Clin Chem, Helsinki 00290, Finland
[2] Univ Helsinki, Dept Med Genet, Helsinki 00290, Finland
[3] Univ Helsinki, Haartman Inst, Helsinki 00290, Finland
[4] Karolinska Hosp, Dept Pathol, S-10401 Stockholm, Sweden
基金
芬兰科学院;
关键词
D O I
10.1016/S0165-4608(97)00341-5
中图分类号
R73 [肿瘤学];
学科分类号
100214 ;
摘要
The antimitotic drug 2-methoxyestradiol is an end metabolite of catechol estrogens. In vivo, it arrests cells in mitosis by interfering with the dynamics of the mitotic spindle without disrupting tubulin formation. It has a mitotic index similar to that of Colcemid in different cell lines. Here we report that 2-methoxyestradiol can be used for making cytogenetic preparations of comparable quality to that of colcemid. In addition, 2-methoxyestradiol is devoid of the toxicity associated with Colcemid, which may make 2-methoxyestradiol useful in slowly growing samples often found in primary solid tumor cultures where a sufficient number of mitotic cells is difficult to obtain. (C) Elsevier Science Inc., 1998.
引用
收藏
页码:139 / 141
页数:3
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