Vascular β-adrenergic receptor adenylyl cyclase system in maturation and aging

The objective of this study was to determine how maturation and aging affects beta (beta)-adrenergic receptor (AR) control of arterial vasorelaxation. Left ventricular (LV) hemodynamics and arterial vasorelaxation in thoracic artery segments were studied in Brown Norway, Fisher 344 cross rats at 6 creeks, 6 months, and 23 months of age. We defined changes in maturation as occurring between 6 weeks and 6 months of age and changes in aging as occurring between 6 months and 23 months of age, With maturation, isoproterenol resulted in a downward shift in heart rate and an upward shift in both LV dP/dt and peripheral vascular resistance responses. Similar changes were noted with aging except for the downward shift in LV dP/dt isoproterenol response. There was a dose-dependent increase in arterial vasorelaxation in response to isoproterenol in all age groups, but the 6-week-old animals had a 5-fold (P<0.01) increase in vasorelaxation compared to other age groups. The isoproterenol-induced arterial vasorelaxation response was not altered by removal of the endothelium. The vasodilatory responses to nitroglycerin, acetylcholine, and adenosine were diminished (P<0.05) with aging. The vasorelaxation responses to forskolin and IBMX were unchanged with maturation and diminished with aging. Incubation of arterial rings in cholera toxin resulted in a reduction in relaxation only in arteries from 6-week-old rats. Maturation resulted in no change in beta-AR density [20.2 +/- 0.7 v 18.5 +/- 0.5 fmol/mg protein, P = N.S.. 6 weeks (n = 2, 18 aortas were combined v 6-month-old rats)]. With maturation, there was no change in G alpha(i) level. However, beta ARK1 levels were increased (55.4 +/- 2.1 v 40.8 +/- 0.4, arbitrary densitometry units) and G alpha(s) levels were decreased (29.5 +/- 0.8 v 49.9 +/- 1.9, arbitrary densitometry units). Aging resulted in no change in beta-AR density (15.3 +/- 1.7 v 18.5 +/- 0.5 fmol/mg membrane protein), but decreases in basal, isoproterenol-. naF-, and forskolin-stimulated AC activities. Compared to 6 week data. 23-month-old rats exhibited no change in either G alpha(i) or beta ARK1, however, G alpha(s) was decreased. In summary, beta-AR-stimulated arterial vasorelaxation is depressed during maturation and aging. Since there is no change in beta-AR density but a decrease in G alpha(s) and in basal/stimulated AC activities, the defect in beta-AR signaling during maturation and aging is probably a post receptor defect, i.e, possibly in the receptor-G protein coupling. (C) 2000 Academic Press.