Production and characterization of a monoclonal antibody against mannose-sensitive hemagglutinin of Vibrio cholerae

被引：10

作者：

Falero, G

Rodríguez, BL

Valmaseda, T

Pérez, ME

Pérez, JL

Fando, R

Robert, A

Campos, J

Silva, A

Sierra, G

Benítez, JA

机构：

[1] Ctr Nacl Invest Cient, Grp Genet, Havana, Cuba

[2] Inst Finlay, Dept Anticuerpos Monoclonales, La Habana, Cuba

[3] Univ La Habana, Dept Microbiol, La Habana, Cuba

来源：

HYBRIDOMA | 1998年 / 17卷 / 01期

关键词：

D O I：

10.1089/hyb.1998.17.63

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

We have generated murine monoclonal antibodies (MAb) against Vibrio cholerae mannose-sensitive hemagglutinin (MSHA) using conventional hybridoma procedures, Seven hybridomas were obtained and one characterized. Hybridoma 2F12/F1 secreted an antibody of the IgG(3) type that reacted with a 17-kDa antigen corresponding to the product of the mshA gene, This MAb inhibited mannose-sensitive agglutination of chicken erythrocytes by EL tor and O139 vibrios, Vibrios expressing MSHA activity inhibited binding of the antibody secreted by 2F12/F1 to MSHA-coated microtiter plates.

引用

页码：63 / 67

页数：5

共 22 条

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