DNA methylation and complete transcriptional silencing of cancer genes persist after depletion of EZH2

被引：89

作者：

McGarvey, Kelly M.

Greene, Eriko

Fahrner, Jill A.

Jenuwein, Thomas

Baylin, Stephen B.

机构：

[1] Johns Hopkins Univ, Sidney Kimmel Comprehens Canc Ctr, Baltimore, MD 21231 USA

[2] Johns Hopkins Univ, Sch Med, Grad Program Cellular & Mol Med, Baltimore, MD 21231 USA

[3] Vienna Bioctr, Res Inst Mol Pathol, Vienna, Austria

来源：

CANCER RESEARCH | 2007年 / 67卷 / 11期

关键词：

D O I：

10.1158/0008-5472.CAN-06-2029

中图分类号：

R73 [肿瘤学];

学科分类号：

100214 ;

摘要：

Recent work suggests a link between the polycomb group protein EZH2 and mediation of gene silencing in association with maintenance of DNA methylation. However, we show that whereas basally expressed target cancer genes with minimal DNA methylation have increased transcription during EZH2 knockdown, densely DNA hypermethylated and silenced genes retain their methylation and remain transcriptionally silent. These results suggest that EZH2 can modulate transcription of basally expressed genes but not silent genes that are densely DNA methylated.

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页码：5097 / 5102

页数：6

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