Determination of papaverine by biotin-avidin amplified ELISA

被引：8

作者：

Yan, J ^{[1
]}

Wang, J ^{[1
]}

Zhao, MP ^{[1
]}

Chang, WB ^{[1
]}

机构：

[1] Beijing Univ, Coll Chem & Mol Engn, Key Lab Bioorgan Chem & Mol Engn, Beijing 100871, Peoples R China

来源：

ANALYTICAL LETTERS | 2004年 / 37卷 / 14期

关键词：

papaverine; biotin; avidin; ELISA; polyclonal antibody; pericarpium papaveris;

D O I：

10.1081/AL-200035851

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

Papaverine [1-(3,4-dimethoxybenzyl)-6,7-dimethoxyisoquinoline; PAP] is one of the most important alkaloids among the naturally occurring opium alkaloids. This work developed an indirect competitive enzyme-linked immunosorbent assay (ELISA) for PAP based on the biotin-avidin amplification system. The linear range of the calibration curve and the limit of detection (defined as three times of the blank) were 0.02-1000 ng/mL and 0.008 ng/mL, respectively. The established method was successfully applied to determining the concentration of PAP in the pericarpium papaveris with the recoveries between 88% and 120%. It was proved to be a highly sensitive and practical method for trace PAP detection compared with the existing gas chromatographymass spectrometry (GC-MS) or high-performance liquid chromatography (HPLC) methods.

引用

页码：2977 / 2989

页数：13

共 22 条

[1] Spectrophotometric determination of meclozine HCl and papaverine HCl in their pharmaceutical formulations [J].