Comparison of real-time PCR detection methods for B1 and P30 genes of Toxoplasma gondii

被引：44

作者：

Buchbinder, S ^{[1
]}

Blatz, R ^{[1
]}

Rodloff, AC ^{[1
]}

机构：

[1] Univ Leipzig, Inst Med Microbiol, D-04103 Leipzig, Germany

来源：

DIAGNOSTIC MICROBIOLOGY AND INFECTIOUS DISEASE | 2003年 / 45卷 / 04期

关键词：

D O I：

10.1016/S0732-8893(02)00549-7

中图分类号：

R51 [传染病];

学科分类号：

100401 ;

摘要：

Real-time PCR assays were established with the Toxoplasma gondii P30 and the 35-copy B1 gene as target genes. Both PCR methods detected Toxoplasma DNA from 10 to 100000 genome equivalents per assay and had comparable intra-assay deviations suggesting that detecting a single copy locus is suitable for clinical diagnostic. (C) 2003 Elsevier Science Inc. All rights reserved.

引用

页码：269 / 271

页数：3

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