Cloning and characterization of rat casein kinase 1ε

被引：57

作者：

Takano, A

Shimizu, K

Kani, S

Buijs, RM

Okada, M

Nagai, K

机构：

[1] Osaka Univ, Inst Prot Res, Div Prot Metab, Suita, Osaka 5650871, Japan

[2] Kobe Univ, Sch Med, Dept Biochem, Kobe, Hyogo 6500017, Japan

[3] Netherlands Inst Brain Res, NL-1105 AZ Amsterdam, Netherlands

来源：

FEBS LETTERS | 2000年 / 477卷 / 1-2期

关键词：

circadian rhythm; differential display; suprachiasmatic nucleus; Per; phosphorylation; nuclear translocation;

D O I：

10.1016/S0014-5793(00)01755-5

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Genes differentially expressed in the subjective day and night in the rat suprachiasmatic nucleus (SCN) were surveyed by differential display. A gene homologous to human casein kinase 1 epsilon(CK1 epsilon) was isolated, which initially appeared to be expressed in the suprachiasmatic nucleus (SCN) in a circadian manner. We here describe the cDNA cloning of the rat CK1 epsilon and characterization of the protein products. The rCK1 epsilon is predominantly expressed in the brain including the SCN, binds and phosphorylates mPer1, mPer2, and mPer3 in vitro, and translocates mPer1 and mPer3, but not mPer2, to the cell nucleus depending on its kinase activity when coexpressed with these Per proteins in COS-7 cells. (C) 2000 Federation of European Biochemical Societies. Published by Elsevier Science B.V. All rights reserved.

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页码：106 / 112

页数：7

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