Purification, cloning, sequencing and over-expression in Escherichia coli of a regioselective aliphatic nitrilase from Acidovorax facilis 72W

被引：49

作者：

Chauhan, S ^{[1
]}

Wu, S ^{[1
]}

Blumerman, S ^{[1
]}

Fallon, RD ^{[1
]}

Gavagan, JE ^{[1
]}

DiCosimo, R ^{[1
]}

Payne, MS ^{[1
]}

机构：

[1] EI Du Pont Nemours & Co, Dept Cent Res & Dev, Expt Stn, Wilmington, DE 19880 USA

来源：

APPLIED MICROBIOLOGY AND BIOTECHNOLOGY | 2003年 / 61卷 / 02期

关键词：

D O I：

10.1007/s00253-002-1192-4

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

A regioselective aliphatic nitrilase from Acidovorax facilis 72W was purified and characterized, and the corresponding gene was cloned and sequenced. This nitrilase gene was over-expressed in Escherichia coli, generating a microorganism that efficiently and regioselectively catalyzes the conversion of aliphatic dinitriles to cyanocarboxylic acids. The high yields obtained, mild reaction conditions used, and robustness observed make this biocatalyst suitable for industrial applications.

引用

页码：118 / 122

页数：5

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