MRNA levels of SREPP-1c do not coincide with the changes in adipose lipogenic gene expression

被引：16

作者：

Bertile, F ^{[1
]}

Raclot, T ^{[1
]}

机构：

[1] Univ Strasbourg, CNRS, Ctr Ecol & Physiol Energet, UPR 9010, F-67087 Strasbourg 2, France

来源：

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS | 2004年 / 325卷 / 03期

关键词：

transcription factors; gene expression; lipid metabolism; regional differences;

D O I：

10.1016/j.bbrc.2004.10.110

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Physiological differences in lipid metabolism exist according to adipose sites. To delineate at which step such gene regulation could occur, mRNA levels of various proteins involved in the overall lipogenic process were determined in subcutaneous (SC) and retroperitoneal (RP) adipose tissues. Fatty acid synthase, malic enzyme, ATP citrate lyase, insulin-sensitive glucose transporter, and glucose-6-phosphate dehydrogenase mRNA levels were coordinately reduced (by up to 50-fold) during fasting in RP and in SC relative to fed rats, and restored or overexpressed (by up to 5- to 6-fold) during refeeding. The response was most often delayed and lower in SC compared to RP. This could contribute to site-specific differences. Interestingly, SREBP-1c mRNA levels were markedly decreased by fasting in SC but remained unchanged in RP. Refeeding tended to restore levels close to fed group values. We conclude that mRNA levels of SREBP-1c do not coincide with the expected changes in adipose lipogenic gene expression of fasted/refed rats. (C) 2004 Elsevier Inc. All rights reserved.

引用

页码：827 / 834

页数：8

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