Taxonomical classification of yeasts isolated from kefir based on the sequence of their ribosomal RNA genes

被引:40
作者
Latorre-Garcia, Lorena
del Castillo-Agudo, Lucas
Polaina, Julio
机构
[1] CSIC, Inst Agroquim & Tecnol Alimentos, E-46100 Valencia, Spain
[2] CSIC, Inst Agroquim & Tecnol Alimentos, E-46980 Valencia, Spain
[3] Univ Valencia, Fac Farm, Dept Microbiol & Ecol, E-46100 Valencia, Spain
关键词
Candida krusei; Issatchenkia orientalis; Kluyveromyces lactis; probiotic; RFLP; Saccharomyces cerevisiae; S; exiguus; humaticus; unisporus;
D O I
10.1007/s11274-006-9298-y
中图分类号
Q81 [生物工程学(生物技术)]; Q93 [微生物学];
学科分类号
071005 ; 0836 ; 090102 ; 100705 ;
摘要
Yeast strains present in 10 samples of kefir of different commercial and domestic origins have been isolated and classified taxonomically on the basis of the internal transcribed sequences (ITS) of their ribosomal RNA genes. A total of 18 yeast strains representing 10 different species have been characterized. Of the three commercial kefir samples analyed, two contained the well characterized yeast Kluyveromyces lactis while no yeast was found in the other one. A broader spectrum of yeast species was found among the home-made kefir samples, of which Issatchenkia orientalis, Saccharomyces unisporus, Saccharomyces exiguus and Saccharomyces humaticus were the most representative species.
引用
收藏
页码:785 / 791
页数:7
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