The role of the cyclic peptide backbone in the anti-HIV activity of the cyclotide kalata B1

被引：112

作者：

Daly, NL

Gustafson, KR

Craik, DJ ^{[1
]}

机构：

[1] Univ Queensland, Inst Mol Biosci, Brisbane, Qld 4072, Australia

[2] NCI, Mol Targets Dev Program, Canc Res Ctr, Frederick, MD 21702 USA

来源：

FEBS LETTERS | 2004年 / 574卷 / 1-3期

基金：

澳大利亚研究理事会;

关键词：

cyclotide; cystine knot; circular protein; acyclic permutant;

D O I：

10.1016/j.febslet.2004.08.007

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The plant cyclotides, the largest known family of circular proteins, have tightly folded structures and a range of biological activities that lend themselves to potential pharmaceutical and agricultural applications. Based on sequence homology, they are classified into the bracelet and Mobius subfamilies. The bracelet subfamily has previously been shown to display anti-HIV activity. We show here that a member of the Mobius subfamily, kalata B1, also exhibits anti-HIV activity despite extensive sequence differences between the subfamilies. In addition, acyclic permutants of kalata B1 displayed no anti-HIV activity, suggesting that this activity is critically dependent on an intact circular backbone. (C) 2004 Federation of European Biochemical Societies. Published by Elsevier B.V. All rights reserved.

引用

页码：69 / 72

页数：4

共 33 条

[1] Solution structure of the cyclotide palicourein: Implications for the development of a pharmaceutical framework [J].