Metabolomic analysis of the consequences of cadmium exposure in Silene cucubalus cell cultures via 1H NMR spectroscopy and chemometrics

被引:95
作者
Bailey, NJC
Oven, M
Holmes, E
Nicholson, JK
Zenk, MH
机构
[1] Univ London Imperial Coll Sci Technol & Med, Div Biomed Sci, London SW7 2AZ, England
[2] Leibniz Inst Pflanzenbiochem, D-06120 Halle Saale, Germany
[3] Univ Halle Wittenberg, Biozentrum, D-06120 Halle Saale, Germany
关键词
cadmium; metabolomics; NMR spectroscopy; Silene cucubalus; metabolite;
D O I
10.1016/S0031-9422(02)00719-7
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
Several essential and non-essential metals (typically those from periods 4, 5 and 6 in groups 11-15 in the periodic table) are commonly detoxified in higher plants by complexation with phytochelatin. The genetic and gross metabolic basis of metal tolerance in plants is, however, poorly understood. Here, we have analyzed plant cell extracts using H-1 NMR spectroscopy combined with multivariate statistical analysis of the data to investigate the biochemical consequences of Cd2+ exposure in Silene cucubalus cell cultures. Principal components analysis of H-1 NMR spectra showed clear discrimination between control and Cd2+ dosed groups, demonstrating the metabolic effects of Cd2+ and thus allowing the identification of increases in malic acid and acetate, and decreases in glutamine and branched chain amino acids as consequences of Cd2+ exposure. This work shows the value of NMR-based metabolomic approaches to the determination of biochemical effects of pollutants in naturally selected populations. (C) 2003 Elsevier Science Ltd. All rights reserved.
引用
收藏
页码:851 / 858
页数:8
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