Kinetics and mechanisms of reactions catalyzed by immobilized lipases

被引：252

作者：

Paiva, AL ^{[1
]}

Balcao, VM ^{[1
]}

Malcata, FX ^{[1
]}

机构：

[1] Univ Catolica Portuguesa, Escola Super Biotecnol, P-4200072 Porto, Portugal

来源：

ENZYME AND MICROBIAL TECHNOLOGY | 2000年 / 27卷 / 3-5期

关键词：

enzymes; structure/function; reaction mechanisms; rate expressions; modeling;

D O I：

10.1016/S0141-0229(00)00206-4

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

This review focuses on the kinetics of several modes of immobilization of lipases, on the mechanisms of reactions of activation of immobilized lipases, and on the kinetics and mechanisms of reactions catalyzed by immobilized lipases. A comprehensive overview of the state of the art pertaining to structural features of lipases is provided as an aid to understand immobilization, interfacial activation, and catalytic performance. General rate expressions are duly derived; more frequent simplifying assumptions are stated and the results thereof listed. Physicochemical and statistical significance of parameters in rate expressions fitted to experimental data are also discussed whenever possible. (C) 2000 Elsevier Science Inc. All rights reserved.

引用

页码：187 / 204

页数：18

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