Effects of a cultured autologous chondrocyte-seeded type II collagen scaffold on the healing of a chondral defect in a canine model

被引:159
作者
Lee, CR
Grodzinsky, AJ
Hsu, HP
Spector, M
机构
[1] Harvard Univ, Brigham & Womens Hosp, Sch Med, Dept Orthopaed Surg, Boston, MA 02115 USA
[2] MIT, Dept Engn Mech, Cambridge, MA 02139 USA
[3] MIT, Dept Elect Engn & Comp Sci, Cambridge, MA 02139 USA
[4] MIT, Biol Engn Div, Cambridge, MA 02139 USA
[5] VA Boston Healthcare Syst, Boston, MA 02130 USA
关键词
D O I
10.1016/S0736-0266(02)00153-5
中图分类号
R826.8 [整形外科学]; R782.2 [口腔颌面部整形外科学]; R726.2 [小儿整形外科学]; R62 [整形外科学(修复外科学)];
学科分类号
摘要
Using a previously established canine model for repair of articular cartilage defects, this study evaluated the 15-week healing of chondral defects (i.e., to the tidemark) implanted with an autologous articular chondrocyte-seeded type 11 collagen scaffold that had been cultured in vitro for four weeks prior to implantation. The amount and composition of the reparative tissue were compared to results from our prior studies using the same animal model in which the following groups were analyzed: defects implanted with autologous chondrocyte-seeded collagen scaffolds that had been cultured in vitro for approximately 12 h prior to implantation, defects implanted with autologous chondrocytes alone, and untreated defects. Chondrocytes, isolated from articular cartilage harvested from the left knee joint of six adult canines, were expanded in number in monolayer for three weeks, seeded into porous type 11 collagen scaffolds, cultured for an additional four weeks in vitro and then implanted into chondral defects in the trochlear groove of the right knee joints. The percentages of specific tissue types filling the defects were evaluated histomorphometrically and certain mechanical properties of the repair tissue were determined. The reparative tissue filled 88 +/- 6% (mean +/- SEM; range 70-100%) of the cross-sectional area of the original defect, with hyaline cartilage accounting for 42 +/- 10% (range 7-67%) of defect area. These values were greater than those reported previously for untreated defects and defects implanted with a type 11 collagen scaffold seeded with autologous chondrocytes within 12 h prior to implantation. Most striking, was the decreased amount of fibrous tissue filling the defects in the current study, 5 +/- 5% (range 0-26%) as compared to previous treatments. Despite this improvement, indentation testing of the repair tissue formed in this study revealed that the compressive stiffness of the repair tissue was well below (20-fold lower stiffness) that of native articular cartilage. (C) 2003 Orthopaedic Research Society. Published by Elsevier Science Ltd. All rights reserved.
引用
收藏
页码:272 / 281
页数:10
相关论文
共 35 条
[1]   VARIATIONS IN THE INTRINSIC MECHANICAL PROTERTIES OF HUMAN ARTICULAR-CARTILAGE WITH AGE, DEGENERATION, AND WATER-CONTENT [J].
ARMSTRONG, CG ;
MOW, VC .
JOURNAL OF BONE AND JOINT SURGERY-AMERICAN VOLUME, 1982, 64 (01) :88-94
[2]   Localized, direct plasmid gene delivery in vivo:: prolonged therapy results in reproducible tissue regeneration [J].
Bonadio, J ;
Smiley, E ;
Patil, P ;
Goldstein, S .
NATURE MEDICINE, 1999, 5 (07) :753-759
[3]   CHANGES IN CARTILAGE COMPOSITION AND PHYSICAL-PROPERTIES DUE TO STROMELYSIN DEGRADATION [J].
BONASSAR, LJ ;
FRANK, EH ;
MURRAY, JC ;
PAGUIO, CG ;
MOORE, VL ;
LARK, MW ;
SANDY, JD ;
WU, JJ ;
EYRE, DR ;
GRODZINSKY, AJ .
ARTHRITIS AND RHEUMATISM, 1995, 38 (02) :173-183
[4]   Autologous chondrocyte implantation in a canine model: change in composition of reparative tissue with time [J].
Breinan, HA ;
Minas, T ;
Hsu, HP ;
Nehrer, S ;
Shortkroff, S ;
Spector, M .
JOURNAL OF ORTHOPAEDIC RESEARCH, 2001, 19 (03) :482-492
[5]   Healing of canine articular cartilage defects treated with microfracture, a type-II collagen matrix, or cultured autologous chondrocytes [J].
Breinan, HA ;
Martin, SD ;
Hsu, HP ;
Spector, M .
JOURNAL OF ORTHOPAEDIC RESEARCH, 2000, 18 (05) :781-789
[6]   Effect of cultured autologous chondrocytes on repair of chondral defects in a canine model [J].
Breinan, HA ;
Minas, T ;
Hsu, HP ;
Nehrer, S ;
Sledge, CB ;
Spector, M .
JOURNAL OF BONE AND JOINT SURGERY-AMERICAN VOLUME, 1997, 79A (10) :1439-1451
[7]  
BREINAN HA, 2001, CLIN ORTHOP S, V391, P219
[8]  
Brittberg M, 1996, CLIN ORTHOP RELAT R, P270
[9]   TREATMENT OF DEEP CARTILAGE DEFECTS IN THE KNEE WITH AUTOLOGOUS CHONDROCYTE TRANSPLANTATION [J].
BRITTBERG, M ;
LINDAHL, A ;
NILSSON, A ;
OHLSSON, C ;
ISAKSSON, O ;
PETERSON, L .
NEW ENGLAND JOURNAL OF MEDICINE, 1994, 331 (14) :889-895
[10]   ARTICULAR-CARTILAGE REPAIR USING ALLOGENEIC PERICHONDROCYTE-SEEDED BIODEGRADABLE POROUS POLYLACTIC ACID (PLA) - A TISSUE-ENGINEERING STUDY [J].
CHU, CR ;
COUTTS, RD ;
YOSHIOKA, M ;
HARWOOD, FL ;
MONOSOV, AZ ;
AMIEL, D .
JOURNAL OF BIOMEDICAL MATERIALS RESEARCH, 1995, 29 (09) :1147-1154