Separate measurement of plasma levels of acylated and desacyl ghrelin in healthy subjects using a new direct ELISA assay

被引:117
作者
Akamizu, T
Shinomiya, T
Irako, T
Fukunaga, M
Nakai, Y
Nakai, Y
Kangawa, K
机构
[1] Kyoto Univ Hosp, Translat Res Ctr, Dept Expt Therapeut, Ghrelin Res Project, Kyoto 6068507, Japan
[2] Kyoto Univ, Fac Med, Sch Hlth Sci, Kyoto 6068507, Japan
[3] Kansai Med Univ, Dept Psychosomat Internal Med, Osaka 5708506, Japan
[4] Natl Cardiovasc Ctr, Res Inst, Dept Biochem, Osaka 5658565, Japan
关键词
D O I
10.1210/jc.2004-1640
中图分类号
R5 [内科学];
学科分类号
1002 ; 100201 ;
摘要
Two forms of ghrelin, acylated and desacyl, circulate in plasma. Although acylation is thought to be essential for ghrelin biological activities, recent studies have suggested that desacyl ghrelin may also possess biological activity. A new commercial ELISA system has now enabled us to measure plasma levels of each of these two ghrelin forms separately. This assay system directly measures levels using small amounts of plasma. To evaluate the utility of this assay system, we measured the plasma levels of the two forms of ghrelin in healthy volunteers. Although acylated ghrelin levels were equivalent to those measured previously by RIA, desacyl ghrelin levels were lower than those expected from the total ghrelin levels previously determined by RIA. The ratios of acylated to desacyl ghrelin significantly correlated with previously determined acylated, but not desacyl, ghrelin levels. After BMI adjustment, the levels of acylated, but not desacyl, ghrelin plasma levels were higher in female subjects than those in males. Several metabolic and hormonal parameters significantly correlated with either plasma acylated or desacyl ghrelin levels. These findings indicate that separate measurements of the two ghrelin form levels may provide valuable information on their structure, gender differences, and physiological implications.
引用
收藏
页码:6 / 9
页数:4
相关论文
共 26 条
  • [1] Delayed short-term secretory regulation of ghrelin in obese animals: Evidenced by a specific RIA for the active form of ghrelin
    Ariyasu, H
    Takaya, K
    Hosoda, H
    Iwakura, H
    Ebihara, K
    Mori, K
    Ogawa, Y
    Hosoda, K
    Akamizu, T
    Kojima, M
    Kangawa, K
    Nakao, K
    [J]. ENDOCRINOLOGY, 2002, 143 (09) : 3341 - 3350
  • [2] Stomach is a major source of circulating ghrelin, and feeding state determines plasma ghrelin-like immunoreactivity levels in humans
    Ariyasu, H
    Takaya, K
    Tagami, T
    Ogawa, Y
    Hosoda, K
    Akamizu, T
    Suda, M
    Koh, T
    Natsui, K
    Toyooka, S
    Shirakami, G
    Usui, T
    Shimatsu, A
    Doi, K
    Hosoda, H
    Kojima, M
    Kangawa, K
    Nakao, K
    [J]. JOURNAL OF CLINICAL ENDOCRINOLOGY & METABOLISM, 2001, 86 (10) : 4753 - 4758
  • [3] Cardiac effects of ghrelin and its endogenous derivatives des-octanoyl ghrelin and des-Gln 14-ghrelin
    Bedendi, I
    Alloatti, G
    Marcantoni, A
    Malan, D
    Catapano, F
    Ghé, C
    Deghenghi, R
    Ghigo, E
    Muccioli, G
    [J]. EUROPEAN JOURNAL OF PHARMACOLOGY, 2003, 476 (1-2) : 87 - 95
  • [4] Non-acylated ghrelin counteracts the metabolic but not the neuroendocrine response to acylated ghrelin in humans
    Broglio, F
    Gottero, C
    Prodam, F
    Gauna, C
    Muccioli, G
    Papotti, M
    Abribat, T
    Van der Lely, AJ
    Ghigo, E
    [J]. JOURNAL OF CLINICAL ENDOCRINOLOGY & METABOLISM, 2004, 89 (06) : 3062 - 3065
  • [5] Identification, characterization, and biological activity of specific receptors for natural (ghrelin) and synthetic growth hormone secretagogues and analogs in human breast carcinomas and cell lines
    Cassoni, P
    Papotti, M
    Ghè, C
    Catapano, F
    Sapino, A
    Graziani, A
    Deghenghi, R
    Reissmann, T
    Ghigo, E
    Muccioli, G
    [J]. JOURNAL OF CLINICAL ENDOCRINOLOGY & METABOLISM, 2001, 86 (04) : 1738 - 1745
  • [6] Ghrelin levels are not regulated by recombinant leptin administration and/or three days of fasting in healthy subjects
    Chan, JL
    Bullen, J
    Lee, JH
    Yiannakouris, N
    Mantzoros, CS
    [J]. JOURNAL OF CLINICAL ENDOCRINOLOGY & METABOLISM, 2004, 89 (01) : 335 - 343
  • [7] A preprandial rise in plasma ghrelin levels suggests a role in meal initiation in humans
    Cummings, DE
    Purnell, JQ
    Frayo, RS
    Schmidova, K
    Wisse, BE
    Weigle, DS
    [J]. DIABETES, 2001, 50 (08) : 1714 - 1719
  • [8] Evaluation of the comparability of commercial ghrelin assays
    Gröschl, M
    Uhr, M
    Kraus, T
    [J]. CLINICAL CHEMISTRY, 2004, 50 (02) : 457 - 458
  • [9] The homeostasis model in the San Antonio Heart Study
    Haffner, SM
    Miettinen, H
    Stern, MP
    [J]. DIABETES CARE, 1997, 20 (07) : 1087 - 1092
  • [10] Ghrelin and des-acyl ghrelin: Two major forms of rat ghrelin peptide in gastrointestinal tissue
    Hosoda, H
    Kojima, M
    Matsuo, H
    Kangawa, K
    [J]. BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, 2000, 279 (03) : 909 - 913