Negative regulation of DNA repair gene (ung) expression by the CpxR/CpxA two-component system in Escherichia coli K-12 and induction of mutations by increased expression of CpxR

被引：15

作者：

Ogasawara, H

Teramoto, J

Hirao, K

Yamamoto, K

Ishihama, A

Utsumi, R

机构：

[1] Kinki Univ, Grad Sch Agr, Dept Biosci & Biotechnol, Nara 6318505, Japan

[2] Nippon Inst Biol Sci, Div Mol Biol, Tokyo, Japan

来源：

JOURNAL OF BACTERIOLOGY | 2004年 / 186卷 / 24期

关键词：

D O I：

10.1128/JB.186.24.8317-8325.2004

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

In Escherichia coli K-12 overexpressing CpxR, transcription of the ung, gene for uracil-DNA glycosylase was repressed, ultimately leading to the induction of mutation. Gel shift, DNase I footprinting, and in vitro transcription assays all indicated negative regulation of ung transcription by phosphorylated CpxR. Based on the accumulated results, we conclude that ung gene expression is negatively regulated by the two-component system of CpxR/CpxA signal transduction.

引用

收藏

页码：8317 / 8325

页数：9

相关论文

共 28 条

[1] CONSTRUCTION OF PLASMIDS CARRYING CI GENE OF BACTERIOPHAGE-LAMBDA [J].

BACKMAN, K ;

PTASHNE, M ;

GILBERT, W .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1976, 73 (11) :4174-4178

[2] A SET OF LACZ MUTATIONS IN ESCHERICHIA-COLI THAT ALLOW RAPID DETECTION OF EACH OF THE 6 BASE SUBSTITUTIONS [J].

CUPPLES, CG ;

MILLER, JH .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1989, 86 (14) :5345-5349

[3] The sigma(E) and the Cpx signal transduction systems control the synthesis of periplasmic protein-folding enzymes in Escherichia coli [J].

Danese, PN ;

Silhavy, TJ .

GENES & DEVELOPMENT, 1997, 11 (09) :1183-1193

[4] One-step inactivation of chromosomal genes in Escherichia coli K-12 using PCR products [J].

Datsenko, KA ;

Wanner, BL .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 2000, 97 (12) :6640-6645

[5] The CpxRA signal transduction system of Escherichia coli:: Growth-related autoactivation and control of unanticipated target operons [J].

De Wulf, P ;

Kwon, O ;

Lin, ECC .

JOURNAL OF BACTERIOLOGY, 1999, 181 (21) :6772-6778

[6] Genome-wide profiling of promoter recognition by the two-component response regulator CpxR-P in Escherichia coli. [J].

De Wulf, P ;

McGuire, AM ;

Liu, XQ ;

Lin, ECC .

JOURNAL OF BIOLOGICAL CHEMISTRY, 2002, 277 (29) :26652-26661

[7] Signal detection and target gene induction by the CpxRA two-component system [J].

DiGiuseppe, PA ;

Silhavy, TJ .

JOURNAL OF BACTERIOLOGY, 2003, 185 (08) :2432-2440

[8] Involvement of the Cpx signal transduction pathway of E-coli in biofilm formation [J].

Dorel, C ;

Vidal, O ;

Prigent-Combaret, C ;

Vallet, I ;

Lejeune, P .

FEMS MICROBIOLOGY LETTERS, 1999, 178 (01) :169-175

[9] SPECIFIC MUTATOR EFFECTS OF UNG (URACIL-DNA GLYCOSYLASE) MUTATIONS IN ESCHERICHIA-COLI [J].

DUNCAN, BK ;

WEISS, B .

JOURNAL OF BACTERIOLOGY, 1982, 151 (02) :750-755

[10] ISOLATION OF INSERTION, DELETION, AND NONSENSE MUTATIONS OF THE URACIL-DNA GLYCOSYLASE (UNG) GENE OF ESCHERICHIA-COLI K-12 [J].

DUNCAN, BK .

JOURNAL OF BACTERIOLOGY, 1985, 164 (02) :689-695