Phenotype-related differential α-2,6-or α-2,3-sialylation of glycoprotein N-glycans in human chondrocytes

被引：39

作者：

Toegel, S. ^{[1
,2
]}

Pabst, M. ^{[3
]}

Wu, S. Q. ^{[4
,5
]}

Grass, J. ^{[3
]}

Goldring, M. B. ^{[2
]}

Chiari, C. ^{[1
]}

Kolb, A. ^{[1
]}

Altmann, F. ^{[3
]}

Viernstein, H. ^{[4
]}

Unger, F. M. ^{[4
]}

机构：

[1] Med Univ Vienna, Dept Orthoped, A-1090 Vienna, Austria

[2] Hosp Special Surg, Weill Cornell Med Coll, Div Res, Lab Cartilage Biol, New York, NY USA

[3] Univ Nat Resources & Appl Life Sci, Dept Chem, Vienna, Austria

[4] Univ Vienna, Dept Pharmaceut Technol & Biopharmaceut, Vienna, Austria

[5] Univ Vienna, Dept Pharmacol & Toxicol, Vienna, Austria

来源：

OSTEOARTHRITIS AND CARTILAGE | 2010年 / 18卷 / 02期

关键词：

Chondrocytes; Extracellular matrix; Cell-matrix interaction; Glycoproteins; Sialic acids; Sialyltransferases; Differentiation; Phenotype; NECROSIS-FACTOR-ALPHA; ARTICULAR-CARTILAGE; GENE-EXPRESSION; COLON ADENOCARCINOMA; LECTIN-BINDING; CELL-SURFACE; TNF-ALPHA; GLYCOSYLATION; CD44; OLIGOSACCHARIDES;

D O I：

10.1016/j.joca.2009.09.004

中图分类号：

R826.8 [整形外科学]; R782.2 [口腔颌面部整形外科学]; R726.2 [小儿整形外科学]; R62 [整形外科学（修复外科学）];

学科分类号：

100224 [整形外科学];

摘要：

Objective Sialic acids frequently occur at the terminal positions of glycoprotein N-glycans present at chondrocyte surfaces or in the cartilage matrix Sialic acids are transferred to glycoproteins in either alpha-2,3 or alpha-2,6 linkage by specific sialyltransferases (SiaTs) and can potentially affect cell functions and cell-matrix interactions The present study aimed to assess the relationship between the expression of the human chondrocyte phenotype and the sialylation of chondrocyte glycoprotein N-glycans. Methods The transcription of 5 SiaT was quantified using real-time Reverse transcription polymerase chain reaction (RT-PCR) assays N-glycan analysis was performed using LC-ESI-MS Primary human chondrocytes were cultured in monolayer or alginate beads and compared to the chondrocyte cell lines C-28/I2 and SW1353. In addition, effects of interteukin-1 beta (IL-1 beta) or tumour necrosis factor-alpha (TNF-alpha) on primary cells were assessed Results Primary human chondrocytes predominantly express alpha-2,6-specific SiaTs and accordingly, alpha-2,6-linked sialic acid residues in glycoprotein N-glycans In contrast, the preponderance of alpha-2,3-linked sialyl residues and, correspondingly, reduced levels of alpha-2,6-specific SiaTs are associated with the altered chondrocyte phenotype of C-28/12 and SW1353 cells Importantly, a considerable shift towards alpha-2,3-linked sialic acids and alpha-2,3-specific SiaT mRNA levels occurred in primary chondrocytes treated with IL-1 beta or tumour necrosis factor-alpha (TNF-alpha) Conclusion The expression of the differentiated chondrocyte phenotype is linked to the ratio of alpha-2,6- to alpha-2,3-linked sialic acids in chondrocyte glycoprotein N-glycans A shift towards altered sialylation might contribute to impaired cell-matrix interactions in disease conditions (C) 2009 Osteoarthntis Research Society International Published by Elsevier Ltd All rights reserved

引用

页码：240 / 248

页数：9

共 61 条

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Improved and simple micro assay for sulfated glycosaminoglycans quantification in biological extracts and its use in skin and muscle tissue studies [J].