Induction of Mycobacterium avium proteins upon infection of human macrophages

被引：11

作者：

Brunori, L

Giannoni, F

Bini, L

Liberatori, S

Frota, C

Jenner, P

Thoresen, OF

Orefici, G

Fattorini, L

机构：

[1] Ist Super Sanita, Dipartimento Malattie Infett Parassitarie & Immun, I-00161 Rome, Italy

[2] Univ Siena, Dept Biol Mol, Funct Proteom Lab, I-53100 Siena, Italy

[3] Natl Inst Med Res, Div Mycobacterial Res, London NW7 1AA, England

来源：

PROTEOMICS | 2004年 / 4卷 / 10期

关键词：

matrix assisted laser desorption; ionization-time of flight-mass spectrometry Mycobacterium avium; reverse transcriptase-polymerase chain reaction THP-1 cells; two-dimensional gel electrophoresis;

D O I：

10.1002/pmic.200300891

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

Induction of Mycobacterium avium proteins labelled with [S-35] methionine and mRNAs upon infection of the human macrophage cell line THP-1 was investigated by two-dimensional gel electrophoresis-mass spectrometry and reverse transcriptase-polymerase chain reaction (RTPCR), respectively. M. avium overexpressed proteins within the macrophages that are involved in fatty acids metabolism (FadE2, FixA), cell wall synthesis (KasA), and protein synthesis (EF-tu). The correlation of differential protein and mRNA expression varied between good and no correlation. Overall, these four proteins may be involved in the adaptation and survival of M. avium within human macrophages.

引用

页码：3078 / 3083

页数：6

共 32 条

[1] A comparison of selected mRNA and protein abundances in human liver [J].