Weight bearing of the limb as a confounding factor in assessment of mechanical allodynia in the rat

被引:54
作者
Kauppila, T
Kontinen, VK
Pertovaara, A [1 ]
机构
[1] Univ Helsinki, Inst Biomed, Dept Physiol, Helsinki, Finland
[2] Univ Helsinki, Inst Biomed, Dept Pharmacol & Toxicol, Helsinki, Finland
[3] Univ Turku, Inst Biomed, Dept Physiol, Turku, Finland
基金
芬兰科学院;
关键词
hindlimb withdrawal threshold; tactile allodynia; nocifensive reflex response;
D O I
10.1016/S0304-3959(97)00143-7
中图分类号
R614 [麻醉学];
学科分类号
100217 ;
摘要
Effect of weight bearing of the hindlimbs on the assessment of mechanically-induced hindlimb withdrawal threshold was determined in intact rats and in rats with various pathophysiological conditions causing allodynia or hyperalgesia. Hindlimb withdrawal was elicited by applying a series of calibrated monofilaments to the plantar or the dorsal surface of the paw. During testing the;at was either in a restraint tube with hindlimbs hanging semi-extended without weight bear,ng or it was standing on a metal grid (bearing its own weight). In intact rats, the withdrawal thresholds were significantly lower when the stimulus site was the dorsal hairy skin rather than the plantar glabrous skin. Also, thresholds were significantly lower when the hindlimbs were not bearing weight. Following carrageenan-induced unilateral inflammation of the plantar paw or a tibial nerve cut ther-was a marked threshold decrease to test stimuli applied to plantar or dorsal paw, respectively, ipsilateral to the pathological condition in standing rats. However, when the hindlimbs were not weight bearing the unilateral threshold decrease was markedly attenuated (carrageenan-treated rats) or completely abolished (tibial cut). In contrast, in rats with a unilateral spinal nerve ligation the threshold decrease ipsilateral to the nerve lesion was highly significant independent of the weight bearing of the hindlimbs. The results indicate that weight bearing of hindlimbs is an important confounding factor in the assessment of tactile allodynia in rats. (C) 1998 International Association for the Study of Pain. Published by Elsevier Science B.V.
引用
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页码:55 / 59
页数:5
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