Genomic amplification and oncogenic properties of the KCNK9 potassium channel gene

被引：205

作者：

Mu, D

Chen, LY

Zhang, XP

See, LH

Koch, CM

Yen, C

Tong, JJ

Spiegel, L

Nguyen, KCQ

Servoss, A

Peng, Y

Pei, L

Marks, JR

Lowe, S

Hoey, T

Jan, LY

McCombie, WR

Wigler, MH

Powers, S

机构：

[1] Tularik Inc, Genom Div, Greenlawn, NY 11740 USA

[2] Cold Spring Harbor Lab, Cold Spring Harbor, NY 11724 USA

[3] Tularik Inc, San Francisco, CA 94080 USA

[4] Duke Univ, Med Ctr, Dept Pathol, Durham, NC 27710 USA

[5] Univ Calif San Francisco, Howard Hughes Med Inst, San Francisco, CA 94143 USA

[6] Univ Calif San Francisco, Dept Physiol, San Francisco, CA 94143 USA

[7] Univ Calif San Francisco, Dept Biochem, San Francisco, CA 94143 USA

来源：

CANCER CELL | 2003年 / 3卷 / 03期

关键词：

D O I：

10.1016/S1535-6108(03)00054-0

中图分类号：

R73 [肿瘤学];

学科分类号：

100214 ;

摘要：

Representational difference analysis (RDA) of human breast cancer was used to discover a novel amplicon located at chromosomal region 8q24.3. We examined a series of breast cancer samples harboring amplification of this region and determined that KCNK9 is the sole overexpressed gene within the amplification epicenter. KCNK9 encodes a potassium channel that is amplified from 3-fold to 10-fold in 10% of breast tumors and overexpressed from 5-fold to over 100-fold in 44% of breast tumors. Overexpression of KCNK9 in cell lines promotes tumor formation and confers resistance to both hypoxia and serum deprivation, suggesting that its amplification and overexpression plays a physiologically important role in human breast cancer.

引用

收藏

页码：297 / 302

页数：6

相关论文

共 25 条

[1] Quantitative mapping of amplicon structure by array CGH identifies CYP24 as a candidate oncogene [J].

Albertson, DG ;

Ylstra, B ;

Segraves, R ;

Collins, C ;

Dairkee, SH ;

Kowbel, D ;

Kuo, WL ;

Gray, JW ;

Pinkel, D .

NATURE GENETICS, 2000, 25 (02) :144-146

[2]

Chapman CG, 2000, MOL BRAIN RES, V82, P74

[3] Positional cloning of ZNF217 and NABC1:: Genes amplified at 20q13.2 and overexpressed in breast carcinoma [J].

Collins, C ;

Rommens, JM ;

Kowbel, D ;

Godfrey, T ;

Tanner, M ;

Hwang, S ;

Polikoff, D ;

Nonet, G ;

Cochran, J ;

Myambo, K ;

Jay, KE ;

Froula, J ;

Cloutier, T ;

Kuo, WL ;

Yaswen, P ;

Dairkee, S ;

Giovanola, J ;

Hutchinson, GB ;

Isola, J ;

Kallioniemi, OP ;

Palazzolo, M ;

Martin, C ;

Ericsson, C ;

Pinkel, D ;

Albertson, D ;

Li, WB ;

Gray, JW .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1998, 95 (15) :8703-8708

[4] Hypoxia-mediated selection of cells with diminished apoptotic potential in solid tumours [J].

Graeber, TG ;

Osmanian, C ;

Jacks, T ;

Housman, DE ;

Koch, CJ ;

Lowe, SW ;

Giaccia, AJ .

NATURE, 1996, 379 (6560) :88-91

[5] LUMEN FORMATION BY EPITHELIAL-CELL LINES IN RESPONSE TO COLLAGEN OVERLAY - A MORPHOGENETIC MODEL IN CULTURE [J].

HALL, HG ;

FARSON, DA ;

BISSELL, MJ .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA-BIOLOGICAL SCIENCES, 1982, 79 (15) :4672-4676

[6] Combined antisense and pharmacological approaches implicate hTASK as an airway O2 sensing K+ channel [J].

Hartness, ME ;

Lewis, A ;

Searle, GJ ;

O'Kelly, I ;

Peers, C ;

Kemp, PJ .

JOURNAL OF BIOLOGICAL CHEMISTRY, 2001, 276 (28) :26499-26508

[7]

Hiemstra J L, 1994, Prog Clin Biol Res, V385, P51

[8] TASK-3, a new member of the tandem pore K+ channel family [J].

Kim, Y ;

Bang, H ;

Kim, D .

JOURNAL OF BIOLOGICAL CHEMISTRY, 2000, 275 (13) :9340-9347

[9] Tissue microarrays for high-throughput molecular profiling of tumor specimens [J].

Kononen, J ;

Bubendorf, L ;

Kallioniemi, A ;

Bärlund, M ;

Schraml, P ;

Leighton, S ;

Torhorst, J ;

Mihatsch, MJ ;

Sauter, G ;

Kallioniemi, OP .

NATURE MEDICINE, 1998, 4 (07) :844-847

[10] Suppression of tumor growth through disruption of hypoxia-inducible transcription [J].

Kung, AL ;

Wang, S ;

Klco, JM ;

Kaelin, WG ;

Livingston, DM .

NATURE MEDICINE, 2000, 6 (12) :1335-1340