High Mobility of Bicoid Captured by Fluorescence Correlation Spectroscopy: Implication for the Rapid Establishment of Its Gradient

被引:83
作者
Abu-Arish, Asmahan [1 ]
Porcher, Aude [3 ]
Czerwonka, Anna [1 ]
Dostatni, Nathalie [3 ]
Fradin, Cecile [1 ,2 ]
机构
[1] McMaster Univ, Dept Phys & Astron, Hamilton, ON L8S 4M1, Canada
[2] McMaster Univ, Dept Biochem & Biomed Sci, Hamilton, ON L8S 4M1, Canada
[3] Inst Curie, Ctr Rech, CNRS, UMR 218, Paris, France
基金
加拿大自然科学与工程研究理事会;
关键词
MORPHOGEN GRADIENT; DROSOPHILA EMBRYO; DIFFUSION; DYNAMICS; PROTEIN; STABILITY; PATTERN; RNA;
D O I
10.1016/j.bpj.2010.05.031
中图分类号
Q6 [生物物理学];
学科分类号
071011 ;
摘要
The Bicoid (Bcd) morphogen is essential for pattern formation in fruit flies. It forms an exponential concentration gradient along the embryo AP axis and turns on cascades of target genes in distinct anterior domains. The most commonly accepted model for gradient formation assumes that Bcd travels by simple diffusion and is uniformly degraded across syncytial embryos, yet several recent studies have challenged these ideas. Here, the question of Bcd mobility was investigated using fluorescence correlation spectroscopy in live Drosophila melanogaster embryos. Bcd-EGFP molecules were found to be highly mobile in the cytoplasm during cycles 12-14, with a diffusion coefficient similar to 7 mu m(2)/s. This value is large enough to explain the stable establishment of the Bcd gradient simply by diffusion before cycle 8, i.e., before the onset of zygotic transcription.
引用
收藏
页码:L33 / L35
页数:3
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