Activated human platelets induce factor XIIa-mediated contact activation

被引:43
作者
Back, Jennie [1 ,2 ,3 ]
Sanchez, Javier [1 ,2 ,3 ]
Elgue, Graciela [1 ,2 ,3 ]
Ekdahl, Kristina Nilsson [1 ,2 ,3 ,4 ]
Nilsson, Bo [1 ,2 ,3 ]
机构
[1] Uppsala Univ, Rudbeck Lab C5, Div Clin Immunol, Dept Oncol, SE-75185 Uppsala, Sweden
[2] Uppsala Univ, Rudbeck Lab C5, Div Clin Immunol, Dept Radiol, SE-75185 Uppsala, Sweden
[3] Uppsala Univ, Rudbeck Lab C5, Div Clin Immunol, Dept Clin Immunol, SE-75185 Uppsala, Sweden
[4] Univ Kalmar, Dept Pure & Appl Nat Sci, SE-39182 Kalmar, Sweden
基金
瑞典研究理事会;
关键词
Antithrombin; Clot formation; Contact activation; Factor XII; Platelets; COAGULATION-FACTOR-XII; IMMOBILIZED HEPARIN; BLOOD PLATELETS; HAGEMAN FACTOR; PLASMA; SURFACE; ANTITHROMBIN; INHIBITION; THROMBOSIS; SYSTEM;
D O I
10.1016/j.bbrc.2009.10.123
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
Earlier studies have shown that isolated platelets in buffer systems can promote activation of FXII or amplify contact activation. in the presence of it negatively charge Substance or material Still proof is lacking that FXII is activated by platelets in a more physiological environment In this study we investigate if activated platelets can induce FXII-mediated contact activation and whether this activation affects clot formation in human blood. Human platelets were activated with a thrombin receptor-activating peptide, SFLLRN-amide, in platelet-rich plasma or in whole blood. FXIIa and FXIa in complex with preferentially antithrombin (AT) and to some extent C1-inhibitor (C1INH) were generated in response to TRAP stimulation. This contact activation was independent of surface-mediated contact activation, tissue factor pathway or thiombin. In clotting whole blood FXIIa-AT and FXIa-AT complexes were specifically formed. demonstrating that AT is a potent inhibitor of FXIIa and FXIa generated by platelet activation Contact activation proteins were analyzed by flow cytometry and FXII, FXI, high-molecular weight kininogen, and prekallikrein were detected oil activated platelets Using chromogenic assays, enzymatic activity of platelet-associated FXIIa, FXIa, and kallikrein were demonstrated Inhibition of FXIIa in non-anticoagulated blood also prolonged the clotting time. We conclude that platelet activation triggers FXII-mediated contact activation oil the Surface and in the vicinity of activated platelets This leads specifically to generation of FXIIa-AT and FXIa-AT complexes, and contributes to clot formation Activated platelets may thereby constitute an intravascular locus for contact activation, which may explain the recently reported importance of FXII in thrombus formation (C) 2009 Published by Elsevier Inc
引用
收藏
页码:11 / 17
页数:7
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