Quantitation of H-pylori cytotoxin mRNA by real-time RT-PCR shows a wide expression range that does not correlate with promoter sequences

被引：9

作者：

Ayala, G

Chihu, L

Perales, G

Fierros-Zárate, G

Hansen, LM

Solnick, JV

Sánchez, J

机构：

[1] Inst Nacl Salud Publ, Ctr Invest Enfermedades Infecciosas, Cuernavaca 62508, Morelos, Mexico

[2] Univ Calif Davis, Dept Internal Med, Ctr Comparat Med, Davis, CA USA

[3] Univ Calif Davis, Dept Med Microbiol, Ctr Comparat Med, Davis, CA USA

[4] Univ Calif Davis, Dept Immunol, Ctr Comparat Med, Davis, CA USA

[5] Univ Autonoma Estado Morelos, Fac Med, Cuernavaca, Morelos, Mexico

来源：

MICROBIAL PATHOGENESIS | 2004年 / 37卷 / 03期

关键词：

RT-PCR; mRNA quantitation; promoter differences; multi-modal gene regulation; Mexican Helicobacter pylori;

D O I：

10.1016/j.micpath.2004.06.003

中图分类号：

R392 [医学免疫学]; Q939.91 [免疫学];

学科分类号：

100102 ;

摘要：

Up to 28-fold differences in vacA expression in Helicobacter pylori strains grown in vitro were demonstrated by real time quantitative RT-PCR. These large differences in expression were unrelated to putative - 35 and - 10 motifs or to other untranslated sequences upstream of the ATG start site. The lack of correlation between promoter sequences and the vacA expression levels suggest the potential existence of a bacterial strain-specific factor, as earlier proposed by others on the basis of reporter gene fusions. (C) 2004 Elsevier Ltd. All rights reserved.

引用

页码：163 / 167

页数：5

共 16 条

[1] MOSAICISM IN VACUOLATING CYTOTOXIN ALLELES OF HELICOBACTER-PYLORI - ASSOCIATION OF SPECIFIC VACA TYPES WITH CYTOTOXIN PRODUCTION AND PEPTIC-ULCERATION [J].