Role of the N-terminal zinc finger of human immunodeficiency virus type 1 nucleocapsid protein in virus structure and replication

被引：119

作者：

Tanchou, V ^{[1
]}

Decimo, D ^{[1
]}

Péchoux, C ^{[1
]}

Lener, D ^{[1
]}

Rogemond, V ^{[1
]}

Berthoux, L ^{[1
]}

Ottmann, M ^{[1
]}

Darlix, JL ^{[1
]}

机构：

[1] Ecole Normale Super Lyon, INSERM, U412, Unite Virol Humaine,LaboRetro, F-69364 Lyon 07, France

来源：

JOURNAL OF VIROLOGY | 1998年 / 72卷 / 05期

关键词：

D O I：

10.1128/JVI.72.5.4442-4447.1998

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

Nucleocapsid protein (NCp7) of human immunodeficiency virus type 1 is found covering the genomic RNA in the interior of the viral particle. It is a highly basic protein with two zinc fingers of the form CX2CX4HX4C which exhibit strong affinity for a zinc cation. To study the structure-function relationship of the N-terminal zinc finger of NCp7, this domain was either deleted or changed to CX2CX4 (C) under bar X4C. We examined virus formation and structure as well as proviral DNA synthesis, Our data show that these two NC mutations result in the formation of particles with an abnormal core morphology and impair the end of proviral DNA synthesis, leading to noninfectious viruses.

引用

页码：4442 / 4447

页数：6

共 51 条

[1] MUTATIONS OF RNA AND PROTEIN SEQUENCES INVOLVED IN HUMAN-IMMUNODEFICIENCY-VIRUS TYPE-1 PACKAGING RESULT IN PRODUCTION OF NONINFECTIOUS VIRUS [J].

ALDOVINI, A ;

YOUNG, RA .

JOURNAL OF VIROLOGY, 1990, 64 (05) :1920-1926

[2] TRANSACTIVATION OF THE MINUS-STRAND DNA TRANSFER BY NUCLEOCAPSID PROTEIN DURING REVERSE TRANSCRIPTION OF THE RETROVIRAL GENOME [J].

ALLAIN, B ;

LAPADATTAPOLSKY, M ;

BERLIOZ, C ;

DARLIX, JL .

EMBO JOURNAL, 1994, 13 (04) :973-981

[3] ANALYSIS OF THE INTERACTIONS OF HIV-1 REPLICATION PRIMER TRANSFER RNA(LYS,3) WITH NUCLEOCAPSID PROTEIN AND REVERSE-TRANSCRIPTASE [J].

BARAT, C ;

SCHATZ, O ;

LEGRICE, S ;

DARLIX, JL .

JOURNAL OF MOLECULAR BIOLOGY, 1993, 231 (02) :185-190

[4] HIV-1 REVERSE-TRANSCRIPTASE SPECIFICALLY INTERACTS WITH THE ANTICODON DOMAIN OF ITS COGNATE PRIMER TRANSFER-RNA [J].

BARAT, C ;

LULLIEN, V ;

SCHATZ, O ;

KEITH, G ;

NUGEYRE, MT ;

GRUNINGERLEITCH, F ;

BARRESINOUSSI, F ;

LEGRICE, SFJ ;

DARLIX, JL .

EMBO JOURNAL, 1989, 8 (11) :3279-3285

[5] Mutations in the N-terminal domain of human immunodeficiency virus type 1 nucleocapsid protein affect virion core structure and proviral DNA synthesis [J].

Berthoux, L ;

Pechoux, C ;

Ottmann, M ;

Morel, G ;

Darlix, JL .

JOURNAL OF VIROLOGY, 1997, 71 (09) :6973-6981

[6] Cyclophilin A is required for an early step in the life cycle of human immunodeficiency virus type 1 before the initiation of reverse transcription [J].

Braaten, D ;

Franke, EK ;

Luban, J .

JOURNAL OF VIROLOGY, 1996, 70 (06) :3551-3560

[7] CORRECT INTEGRATION OF RETROVIRAL DNA INVITRO [J].

BROWN, PO ;

BOWERMAN, B ;

VARMUS, HE ;

BISHOP, JM .

CELL, 1987, 49 (03) :347-356

[8]

CARRIERE C, 1995, J VIROL, V69, P2366

[9] Human immunodeficiency virus type 1 nucleocapsid protein specifically stimulates Mg2+-dependent DNA integration in vitro [J].

Carteau, S ;

Batson, SC ;

Poljak, L ;

Mouscadet, JF ;

DeRocquigny, H ;

Darlix, JL ;

Roques, BP ;

Kas, E ;

Auclair, C .

JOURNAL OF VIROLOGY, 1997, 71 (08) :6225-6229

[10] FUSION FROM WITHOUT DIRECTED BY HUMAN-IMMUNODEFICIENCY-VIRUS PARTICLES [J].

CLAVEL, F ;

CHARNEAU, P .

JOURNAL OF VIROLOGY, 1994, 68 (02) :1179-1185

← 1 2 3 4 5 6 →