Multiplex PCR for detection of three plasmid-borne genes of enteroaggregative Escherichia coli strains

被引：116

作者：

Cerna, JF

Nataro, JP

Estrada-Garcia, T

机构：

[1] Inst Politecn Nacl, CINVESTAV, Dept Mol Biomed, Mexico City 07360, DF, Mexico

[2] Univ Maryland, Sch Med, Dept Pediat & Med, Ctr Vaccine Dev, Baltimore, MD 21201 USA

来源：

JOURNAL OF CLINICAL MICROBIOLOGY | 2003年 / 41卷 / 05期

关键词：

D O I：

10.1128/JCM.41.5.2138-2140.2003

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

We developed a novel multiplex PCR assay for enteroaggregative Escherichia coli (EAEC) detection, by using three plasmid-borne genes (the aggregative adherence [AA] probe, aap, and aggR). One or more of the loci were detected in 24 (86%) of 28 patient isolates analyzed. The multiplex PCR assay is a fast, convenient, and sensitive molecular test to detect EAEC.

引用

页码：2138 / 2140

页数：3

共 24 条

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