Bystander-killing effect and cyclic induction of TNF-α gene under heat-inducible promoter gadd 153

被引：5

作者：

Ito, A ^{[1
]}

Shinkai, M ^{[1
]}

Bouhon, IA ^{[1
]}

Honda, H ^{[1
]}

Kobayashi, T ^{[1
]}

机构：

[1] Nagoya Univ, Grad Sch Engn, Dept Biotechnol, Nagoya, Aichi 4648603, Japan

来源：

JOURNAL OF BIOSCIENCE AND BIOENGINEERING | 2000年 / 90卷 / 04期

基金：

日本学术振兴会;

关键词：

TNF-alpha; bystander effect; stress-inducible promoter; gadd; 153; glioma; hyperthermia;

D O I：

10.1016/S1389-1723(01)80015-3

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

The bystander-killing effect and cyclic induction of tumor necrosis factor (TNF)-alpha gene expression under the heat-inducible promoter gadd 153 (growth arrest and DNA damage) were investigated. The plasmid harboring the TNF-alpha gene under the control of the gadd 153 promoter (pGadTNF) was introduced into cells of the human glioma cell line U251-SP. When the transfected cells were exposed to heat treatment, TNF-alpha gene expression was induced. The heated pGadTNF-transfected cells were co-cultured the nonheated pGadTNF-transfected cells as bystander cells. As a result, TNF-alpha gene expression in the bystander cells was observed. The level of TNF-alpha gene expression was further enhanced in the co-cultured cells. Due to this cyclic induction, a strong cytotoxic effect on the bystander cells was observed. Based on this bystander-killing effect, it was concluded that the transfection of the TNF-alpha gene under the control of the gadd 153 promoter into tumor cells combined with hyperthermia would be a potent tool for cancer therapy.

引用

页码：437 / 441

页数：5

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