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The use of recombinant antibodies in proteomics

被引：91
作者：
Holt, LJ
Enever, C
de Wildt, RMT
Tomlinson, IM
机构：
[1] MRC, Mol Biol Lab, Cambridge CB2 2QH, England
[2] MRC, Ctr Prot Engn, Cambridge CB2 2QH, England
来源：
CURRENT OPINION IN BIOTECHNOLOGY | 2000年 / 11卷 / 05期
关键词：
D O I：
10.1016/S0958-1669(00)00133-6
中图分类号：
Q5 [生物化学];
学科分类号：
071010 ; 081704 ;
摘要：
Recombinant antibodies are becoming increasingly important in the field of proteomics. Recent advances include the development of large phage-antibody libraries that contain high-affinity binders to almost any target protein, and new methods for high-throughput selection of antibody-antigen interactions. Coupled with a range of new screening technologies that use high-density antibody arrays to identify differentially expressed proteins, these antibody libraries can be applied to whole proteome analysis.
引用
收藏
页码：445 / 449
页数：5
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