Feedback inhibition on cell wall integrity signaling by Zds1 involves Gsk3 phosphorylation of a cAMP-dependent protein kinase regulatory subunit

被引:41
作者
Griffioen, G
Swinnen, S
Thevelein, JM
机构
[1] Katholieke Univ Leuven, Mol Cell Biol Lab, Inst Bot & Microbiol, B-3001 Louvain, Flanders, Belgium
[2] VIB, Dept Mol Microbiol, B-3001 Louvain, Flanders, Belgium
关键词
D O I
10.1074/jbc.M210691200
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
We report here that budding yeast cAMP-dependent protein kinase (cAPK) is controlled by heat stress. A rise in temperature from 30 to 37degreesC was found to result in both a higher expression and an increased cytoplasmic localizationof its regulatory subunit Bcy1. Both of these effects required phosphorylationof serines located in its localization domain. Surprisingly, classic cAPK-controlled processes were found to be independent of Bcy1 phosphorylation, indicating that these modifications do not affect cAPK activity as such. Alternatively, phosphorylation may recruit cAPK to, and thereby control, a specific subsetof ( perhaps novel) cAPK targets that are presumably localized extranuclearly. Zds1 and Zds2 may play a role in this process, since these were found required to retain hyperphosphorylated Bcy1 in the cytoplasm at 37degreesC. Mck1, a homologue of mammalian glycogen synthase kinase 3 and a downstream component ofthe heat-activated Pkc1-Slt2/Mpk1 cell wall integrity pathway, is partly responsible for hyperphosphorylations of Bcy1. Remarkably, Zds1 appears to act as a negative regulator of cell wall integrity signaling, and this activity is dependent in part on the phosphorylation status of Bcy1. Thus, Mck1 phosphorylation of Bcy1 and Zds1 may constitute an unprecedented negative feedback control on the cell wall integrity-signaling pathway.
引用
收藏
页码:23460 / 23471
页数:12
相关论文
共 51 条
[1]  
Bi EF, 1996, MOL CELL BIOL, V16, P5264
[2]   PROPERTIES OF SACCHAROMYCES-CEREVISIAE WEE1 AND ITS DIFFERENTIAL REGULATION OF P34(CDC28) IN RESPONSE TO G(1) AND G(2) CYCLINS [J].
BOOHER, RN ;
DESHAIES, RJ ;
KIRSCHNER, MW .
EMBO JOURNAL, 1993, 12 (09) :3417-3426
[3]  
Carlson CR, 2001, J CELL SCI, V114, P3243
[4]   HOW MAP KINASES ARE REGULATED [J].
COBB, MH ;
GOLDSMITH, EJ .
JOURNAL OF BIOLOGICAL CHEMISTRY, 1995, 270 (25) :14843-14846
[5]   AKAPs: from structure to function [J].
Colledge, M ;
Scott, JD .
TRENDS IN CELL BIOLOGY, 1999, 9 (06) :216-221
[6]   A SYNTHETIC LETHAL SCREEN IDENTIFIES SLK1, A NOVEL PROTEIN-KINASE HOMOLOG IMPLICATED IN YEAST-CELL MORPHOGENESIS AND CELL-GROWTH [J].
COSTIGAN, C ;
GEHRUNG, S ;
SNYDER, M .
MOLECULAR AND CELLULAR BIOLOGY, 1992, 12 (03) :1162-1178
[7]  
DAVENPORT KR, 1995, J BIOL CHEM, V270, P30157
[8]   NEW YEAST-ESCHERICHIA-COLI SHUTTLE VECTORS CONSTRUCTED WITH INVITRO MUTAGENIZED YEAST GENES LACKING 6-BASE PAIR RESTRICTION SITES [J].
GIETZ, RD ;
SUGINO, A .
GENE, 1988, 74 (02) :527-534
[9]   A role for the Pkc1 MAP kinase pathway of Saccharomyces cerevisiae in bud emergence and identification of a putative upstream regulator [J].
Gray, JV ;
Ogas, JP ;
Kamada, Y ;
Stone, M ;
Levin, DE ;
Herskowitz, I .
EMBO JOURNAL, 1997, 16 (16) :4924-4937
[10]   Nutritional control of nucleocytoplasmic localization of cAMP-dependent protein kinase catalytic and regulatory subunits in Saccharomyces cerevisiae [J].
Griffioen, G ;
Anghileri, P ;
Imre, E ;
Baroni, MD ;
Ruis, H .
JOURNAL OF BIOLOGICAL CHEMISTRY, 2000, 275 (02) :1449-1456