Sugar uptake and transport in rice embryo.: Expression of companion cell-specific sucrose transporter (OsSUT1) induced by sugar and light

被引:117
作者
Matsukura, C
Saitoh, T
Hirose, T
Ohsugi, R
Perata, P
Yamaguchi, J [1 ]
机构
[1] Nagoya Univ, Biosci Ctr, Chikusa Ku, Nagoya, Aichi 4648601, Japan
[2] Nagoya Univ, Grad Sch Bioagr Sci, Chikusa Ku, Nagoya, Aichi 4648601, Japan
[3] Natl Inst Agrobiol Resources, Dept Plant Physiol, Tsukuba, Ibaraki 305, Japan
[4] Univ Bari, Dipartimento Biol Patol Vegetale, I-70125 Bari, Italy
关键词
D O I
10.1104/pp.124.1.85
中图分类号
Q94 [植物学];
学科分类号
071001 ;
摘要
We investigated sugar uptake and transport in rice (Oryza sativa) embryo during grain germination. Endogenous sugar levels, accumulation of starch granules, and gene expression of a rice sucrose transporter (OsSUT1) were examined using rice embryos germinated with or without exogenous sugar supply. Starch granules remarkably accumulated in the cells around vascular bundles as a consequence of the sugar taken up by the embryos, indicating that the taken-up sugars are transiently converted into starch. In situ detection for OsSUT1 mRNA indicated its localization in the phloem companion cells. Furthermore, northern-blot and in situ hybridization analyses showed that OsSUT1 expression is not detectable in embryos subjected to sugar starvation conditions, whereas its expression is enhanced by an increased endogenous sugar level. Overall results indicate that the expression of companion cell-specific sucrose transporter, OsSUT1 is regulated by the endogenous sugar status as well as light exposure.
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页码:85 / 93
页数:9
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