Effect of silver nanoparticles on the electron transfer reactivity and the catalytic activity of myoglobin

被引:118
作者
Gan, X
Liu, T
Zhong, J
Liu, XJ
Li, GX [1 ]
机构
[1] Nanjing Univ, Dept Biochem, Nanjing 210093, Peoples R China
[2] Nanjing Univ, Natl Key Lab Pharmaceut Biotechnol, Nanjing 210093, Peoples R China
关键词
electrochemistry; electrodes; myoglobin; nanoparticles;
D O I
10.1002/cbic.200400080
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
Silver nanoparticles (11 +/- 1.5 nm) could greatly enhance the electron-transfer reactivity of myoglobin (Mb) and its catalytic ability toward hydrogen peroxide (H2O2). Direct fast electron transfer between Mb and a pyrolytic graphite (PG) electrode was achieved, and a pair of well-defined, quasireversible redox peaks was obtained. The cathodic and anodic peaks were located at -329 and -281 mV, respectively. Meanwhile, the catalytic ability of the protein toward the reduction of H2O2 was also studied, and a H2O2 biosensor was subsequently fabricated. Its detection limit Was 1.0 x 10(-6) M with a sensitivity of 0.0205 muA per muM of H2O2, The apparent Michaelis-Menten constant was calculated to be 1303 muM. Flocculation assay showed that the protein maintained plasmon layers surrounding the surface of silver nonoparticles and avoided silver-nanoparticle aggregation. On the other hand, UV-visible spectroscopy studies revealed that silver nanoparticles could induce a small change of the heme-group environment of the protein; this contributed to the enhancement of the electron-transfer reactivity and the catalytic activity.
引用
收藏
页码:1686 / 1691
页数:6
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