Caveolin-1 influences P2X7 receptor expression and localization in mouse lung alveolar epithelial cells

被引：54

作者：

Barth, K.

Weinhold, K.

Guenther, A.

Young, M. T.

Schnittler, H.

Kasper, M.

机构：

[1] Tech Univ Dresden, Med Fac Carl Gustav Carus, Inst Anat, D-01307 Dresden, Germany

[2] Univ Manchester, Fac Life Sci, Manchester, Lancs, England

[3] Tech Univ Dresden, Med Fac Carl Gustav Carus, Inst Physiol, Dresden, Germany

来源：

FEBS JOURNAL | 2007年 / 274卷 / 12期

关键词：

alveolar epithelium; Caveolin-1; mouse lung; P2X7; receptor;

D O I：

10.1111/j.1742-4658.2007.05830.x

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The P2X(7) receptor has recently been described as a marker for lung alveolar epithelial type I cells. Here, we demonstrate both the expression of P2X(7) protein and its partition into lipid rafts in the mouse lung alveolar epithelial cell line E10. A significant degree of colocalization was observed between P2X(7) and the raft marker protein Caveolin-1; also, P2X(7) protein was associated with caveolae. A marked reduction in P2X(7) immunoreactivity was observed in lung sections prepared from Caveolin-1-knockout mice, indicating that Caveolin-1 expression was required for full expression of P2X(7) protein. Indeed, suppression of Caveolin-1 protein expression in E10 cells using short hairpin RNAs resulted in a large reduction in P2X(7) protein expression. Our data demonstrate a potential interaction between P2X(7) protein and Caveolin-1 in lipid rafts, and provide a basis for further functional and biochemical studies to probe the physiologic significance of this interaction.

引用

页码：3021 / 3033

页数：13

共 48 条

[1] Tyrosine phosphorylation of HSP90 within the P2X7 receptor complex negatively regulates P2X7 receptors [J].