The expression of calcium/calmodulin-dependent protein kinase II-α in the hippocampus of patients with Alzheimer's disease and its links with AD-related pathology

被引:65
作者
Wang, YJ
Chen, GH
Hu, XY
Lu, YP
Zhou, HN
Liu, RY [1 ]
机构
[1] Anhui Med Univ, Affiliated Hosp 1, Dept Geriatr, Hefei 230022, Peoples R China
[2] Univ Sci & Technol China, Sch Life Sci, Dept Neurobiol, Hefei 230026, Peoples R China
关键词
Alzheimer's disease; hippocampus; calcium/calmodulin-dependent protein kinase II-alpha; hyperphosphorylated tau; beta-amyloid; senile plaques;
D O I
10.1016/j.brainres.2004.10.061
中图分类号
Q189 [神经科学];
学科分类号
071006 ;
摘要
Alzheimer's disease (AD) is characterized pathologically by selective neuronal loss and by the formation of neurofibrillary tangles (NFTs) and senile plaques (SPs). Since calcium/calmodulin-dependent protein kinase II-alpha (CaMKII-alpha), one of the most abundant kinases in the brain, is involved in the phosphorylation of tau and amyloid precursor protein (APP), we examined the expression of CaMKII-alpha and its relationships with the neuropathology in the hippocampus of AD patients using immunohistochemistry and double-labeling immunofluorescence methods. The results showed that CaMKII-alpha containing neurons were selectively lost in the CA1 subfield of AD hippocampus and accompanied with enhanced immunoreactivity in the remaining neurons. About 33% hyperphosphorylated tau-containing neurons labeled by monoclonal, antibody AT-8 were also immunoreactive for CaMKII-alpha. Moreover, we found for the first time that the immunoreactivity of CaMKII-alpha was largely deposited in the SPs of the AD hippocampus. The pattern of the co-localization of CaMKII-alpha with beta amyloid depended on the type of SPs. Since the co-localization of CaMKII-alpha with hyperphosphorylated tau is relatively rare, we concluded that CaMKII-alpha may be related with beta-amyloid more closely than being involved in tau hyperphosphorylation. (C) 2004 Elsevier B.V. All rights reserved.
引用
收藏
页码:101 / 108
页数:8
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