PCR detection of hemolysin (vhh) gene in Vibrio harveyi

被引：38

作者：

Conejero, MJU ^{[1
]}

Hedreyda, CT ^{[1
]}

机构：

[1] Univ Philippines, Natl Inst Mol Biol & Biotechnol UP Diliman, Mol Microbiol Lab, Quezon City 1101, Philippines

来源：

JOURNAL OF GENERAL AND APPLIED MICROBIOLOGY | 2004年 / 50卷 / 03期

关键词：

hemolysin; PCR; vhh; Vibrio harveyi;

D O I：

10.2323/jgam.50.137

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

The Vibrio harveyi hemolysin gene (vhh), which encodes for a virulence factor involved in pathogenicity to fish and shellfish species, may be targeted for species detection or strain differentiation. Primers designed for this gene were used in detection studies of V harveyi strains from various hosts. One primer set among four tested, could amplify the expected gene fragment in PCR using templates from all 11 V harveyi strains studied. Detection of the presence of the hemolysin gene could therefore serve as a suitable detection marker of Vibrio harveyi isolates potentially pathogenic to fish and shrimps.

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页码：137 / 142

页数：6

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