Amperometric biosensors for L-alanine and pyruvate assays in biological fluids

被引：17

作者：

Gilis, M ^{[1
]}

Durliat, H ^{[1
]}

Comtat, M ^{[1
]}

机构：

[1] Univ Toulouse 3, CNRS, UMR 5503, Lab Genie Chim, F-31062 Toulouse, France

来源：

ANALYTICA CHIMICA ACTA | 1997年 / 355卷 / 2-3期

关键词：

biosensors; amperometry; L-alanine; pyruvate; blood; fermentation;

D O I：

10.1016/S0003-2670(97)00491-1

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

Two amperometric electrochemical biosensors have been developed and used to determine the concentrations of L-alanine and pyruvate in biological fluids (blood, fermentation media) without any prior treatment of the sample. The proposed biosensors have response times lower than 5 min, a precision of 2% and the measured current is proportional to the substrate concentration in the range from 1 x 10(-6) to 2 x 10(-3) mol l(-1). They have an operating life of one month. (C) 1997 Elsevier Science B.V.

引用

页码：235 / 240

页数：6

共 18 条

[1]

ADACHI S, 1984, BIOTECH BIOENGB, V26, p63P5

[2] THE DEVELOPMENT OF A HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHIC ASSAY-METHOD TO DETERMINE PYRUVATE GENERATED DURING THE C-S LYSIS OF CYSTEINE CONJUGATED XENOBIOTICS [J].

BUCKBERRY, LD ;

GALLAGHER, SD ;

SHAW, PN .

JOURNAL OF LIQUID CHROMATOGRAPHY, 1993, 16 (14) :3073-3081

[3] APPLICATION OF LIGAND-EXCHANGE CHROMATOGRAPHY TO THE ASSAY OF L-ALANINE FROM DL-ASPARTIC ACID BY PSEUDOMONAS-DACUNHAE [J].

CHENG, CY ;

HUANG, IH .

JOURNAL OF CHROMATOGRAPHY, 1991, 555 (1-2) :272-277

[4] OPTIMAL CONDITIONS FOR FUNCTIONING OF SPECIFIC LACTATE ENZYME ELECTRODE SYSTEM - APPLICATION TO DETERMINATION OF LACTATE IN BLOOD [J].

DURLIAT, H ;

COMTAT, M ;

MAHENC, J ;

BAUDRAS, A .

ANALYTICA CHIMICA ACTA, 1976, 85 (01) :31-40

[5]

FURST P, 1987, INFUSIONSTHERAPIE, V14, P137

[6]

Gilis M, 1996, AM J ENOL VITICULT, V47, P11

[7]

KARL IE, 1972, J LAB CLIN MED, V80, P424

[8]

KOIKE K, 1990, J CLIN BIOCHEM NUTR, V9, P151

[9] APPLICATION OF A FLOW-INJECTION FIBER OPTIC BIOSENSOR FOR THE ANALYSIS OF DIFFERENT AMINO-ACIDS [J].

LEE, SJ ;

SCHEPER, T ;

BUCKMANN, AF .

BIOSENSORS & BIOELECTRONICS, 1994, 9 (01) :29-32

[10]

LLOYD B, 1978, CLIN CHEM, V24, P1724

← 1 2 →