Development and application of a pig IL-8 ELISA detection system

被引：8

作者：

Splichal, I ^{[1
]}

Muneta, Y

Mori, Y

Takahashi, E

机构：

[1] Acad Sci Czech Republ, Dept Immunol & Gnotobiol, Inst Microbiol, Novy Hradek 54922, Czech Republic

[2] Univ Tokyo, Dept Vet Microbiol, Grad Sch Agr & Life Sci, Tokyo, Japan

[3] Natl Inst Anim Hlth, Dept Immunol, Tsukuba, Ibaraki 305, Japan

来源：

JOURNAL OF IMMUNOASSAY & IMMUNOCHEMISTRY | 2003年 / 24卷 / 02期

基金：

日本学术振兴会;

关键词：

ELISA; interleukin; 8; chemokine; monoclonal antibody; pig;

D O I：

10.1081/IAS-120020086

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

Interleukin 8 (IL-8) is a chemotactic and activating chemokine, especially for neutrophils, which plays an important role in inflammatory process. A pig IL-8 specific enzyme-linked immunosorbent assay (ELISA) was developed to measure IL-8 concentrations in cell culture supernatants and biological fluids. A streptavidin-biotin amplified sandwich method uses mouse capture mAb IZ8.03 and detection biotinylated mouse mAb IZ8.04 against recombinant pig IL-8. The assay specifically and reproducibly recognizes both recombinant and natural pig IL-8. A working range of the assay is 16-1000 pg/mL and takes a mere 3.5 h of incubation time. This pig IL-8 ELISA is a suitable alternative way of measurement of IL-8 concentrations to time consuming and laborious IL-8 bioassays.

引用

页码：219 / 232

页数：14

共 31 条

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